Cluster analysis of WIBS single-particle bioaerosol data
Robinson, Niall H.
Huffman, J. A.
Kaye, Paul H.
Hierarchical agglomerative cluster analysis was performed on single-particle multi-spatial data sets comprising optical diameter, asymmetry and three different ﬂuorescence measurements, gathered using two dual Wideband Integrated Bioaerosol Sensors (WIBSs). The technique is demonstrated on measurements of various ﬂuorescent and non-ﬂuorescent polystyrene latex spheres (PSL) before being applied to two separate contemporaneous ambient WIBSdata sets recorded in a forest site in Colorado, USA, as part of the BEACHON-RoMBAS project. Cluster analysis results between both data sets are consistent. Clusters are tentatively interpreted by comparison of concentration time series and cluster average measurement values to the published literature (of which there is a paucity) to represent the following: non-ﬂuorescent accumulation mode aerosol; bacterial agglomerates; and fungal spores. To our knowledge, this is the ﬁrst time cluster analysis has been applied to long-term online primary biological aerosol particle (PBAP) measurements. The novel application of this clustering technique provides a means for routinely reducing WIBS data to discrete concentration time series which are more easily interpretable, without the need for any a priori assumptions concerning the expected aerosol types. It can reduce the level of subjectivity compared to the more standard analysis approaches, which are typically performed by simple inspection of various ensemble data products. It also has the advantage of potentially resolving less populous or subtly different particle types. This technique is likely to become more robust in the future as ﬂuorescence-based aerosol instrumentation measurement precision, dynamic range and the number of available metrics are improved.