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dc.contributor.authorUnderwood, Sarah
dc.contributor.authorGuan, Shuang
dc.contributor.authorVijayasubhash, Vinod
dc.contributor.authorBaines, Simon D.
dc.contributor.authorGraham, Luke
dc.contributor.authorLewis, Richard J.
dc.contributor.authorWilcox, M. H.
dc.contributor.authorStephenson, Keith
dc.identifier.citationUnderwood , S , Guan , S , Vijayasubhash , V , Baines , S D , Graham , L , Lewis , R J , Wilcox , M H & Stephenson , K 2009 , ' Characterization of the sporulation initiation pathway of Clostridium difficile and its role in toxin production ' , Journal of Bacteriology , vol. 191 , no. 23 , pp. 7296-305 .
dc.identifier.otherPURE: 1351782
dc.identifier.otherPURE UUID: a7503356-24d1-4810-9d67-4d3e630bd060
dc.identifier.otherPubMed: 19783633
dc.identifier.otherScopus: 71749105366
dc.description.abstractClostridium difficile is responsible for significant mortality and morbidity in the hospitalized elderly. C. difficile spores are infectious and are a major factor contributing to nosocomial transmission. The Spo0A response regulator is the master regulator for sporulation initiation and can influence many other cellular processes. Using the ClosTron gene knockout system, we inactivated genes encoding Spo0A and a putative sporulation-associated sensor histidine kinase in C. difficile. Inactivation of spo0A resulted in an asporogeneous phenotype, whereas inactivation of the kinase reduced C. difficile sporulation capacity by 3.5-fold, suggesting that this kinase also has a role in sporulation initiation. Furthermore, inactivation of either spo0A or the kinase resulted in a marked defect in C. difficile toxin production. Therefore, Spo0A and the signaling pathway that modulates its activity appear to be involved in regulation of toxin synthesis in C. difficile. In addition, Spo0A was directly phosphorylated by a putative sporulation-associated kinase, supporting the hypothesis that sporulation initiation in C. difficile is controlled by a two-component signal transduction system rather than a multicomponent phosphorelay. The implications of these findings for C. difficile sporulation, virulence, and transmission are discussed.en
dc.relation.ispartofJournal of Bacteriology
dc.titleCharacterization of the sporulation initiation pathway of Clostridium difficile and its role in toxin productionen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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