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dc.contributor.authorFreeman, J.
dc.contributor.authorWard, Elaine
dc.contributor.authorCalderon, C.
dc.contributor.authorMcCartney, A.
dc.identifier.citationFreeman , J , Ward , E , Calderon , C & McCartney , A 2002 , ' A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum ' , European Journal of Plant Pathology , vol. 108 , no. 9 , pp. 877-886 .
dc.identifier.otherPURE: 1270192
dc.identifier.otherPURE UUID: d579d3de-8602-47a0-8fa1-b2e8a1407dc2
dc.identifier.otherWOS: 000179496800006
dc.identifier.otherScopus: 0036867329
dc.description.abstractThe development of a polymerase chain reaction (PCR) assay for the detection of inoculum of the plant pathogenic fungus Sclerotinia sclerotiorum is described. The PCR primers were designed using nuclear ribosomal DNA internal transcribed spacer sequences. Specific detection of DNA from S. sclerotiorum was possible even in the presence of a 40-fold excess of DNA from the closely related fungus Botrytis cinerea. PCR products were obtained from suspensions of untreated S. sclerotiorum ascospores alone, but DNA purification was required for detection in the presence of large numbers of B. cinerea conidiospores. Specific detection of inoculum of S. sclerotiorum was possible in field-based air-samples, using a Burkard spore trap, and from inoculated oilseed rape petals. The assay has potential for incorporation into a risk management system for S. sclerotiorum in oilseed rape cropsen
dc.relation.ispartofEuropean Journal of Plant Pathology
dc.titleA polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorumen
dc.contributor.institutionCrop and Environmental Protection
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionGeography, Environment and Agriculture
dc.description.statusPeer reviewed
rioxxterms.typeJournal Article/Review

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