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        Determination of halofuginone in bovine plasma by competing-ion high performance liquid chromatography after solid phase extraction

        Author
        Kinabo, L. D.
        McKellar, Quintin
        Murray, M.
        Attention
        2299/10984
        Abstract
        A high performance liquid chromatography (HPLC) method for the determination of the anticoccidial and antitheilerial drug halofuginone in bovine plasma was developed. Samples were diluted with acetic acid (10%, v/v) and cleaned up on a Bond Elut C8 column. The analyte was eluted from the extraction column and chromatographed by reversed-phase HPLC using decylamine as a competing-ion reagent. Detection was by UV at 243 nm. Recovery from plasma was 75%, and within-day and between-day coefficients of variation were 5.23 and 6.35% respectively. The specificity and sensitivity of this method (limit of detection in plasma, 1 ng/mL) were sufficiently high to enable us to characterize the time course of the drug in plasma after oral administration of therapeutic doses to cattle.
        Publication date
        1989
        Published in
        Biomedical Chromatography
        Published version
        https://doi.org/10.1002/bmc.1130030310
        Other links
        http://hdl.handle.net/2299/10984
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