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Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

dc.contributor.authorKozlakidis, Z.
dc.contributor.authorCitir, A.
dc.contributor.authorAcikgoz, S.
dc.contributor.authorCoutts, Robert H.A.
dc.date.accessioned2013-09-02T12:45:03Z
dc.date.available2013-09-02T12:45:03Z
dc.date.issued2007-12
dc.identifier.citationKozlakidis , Z , Citir , A , Acikgoz , S & Coutts , R H A 2007 , ' Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease ' , Plant Pathology , vol. 56 , no. 6 , pp. 1032-1035 . https://doi.org/10.1111/j.1365-3059.2007.01690.x
dc.identifier.issn0032-0862
dc.identifier.urihttp://hdl.handle.net/2299/11473
dc.description.abstractA reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.en
dc.format.extent4
dc.language.isoeng
dc.relation.ispartofPlant Pathology
dc.subjectDOUBLE-STRANDED RNAS
dc.subjectamasya cherry disease
dc.subjectdsRNAs
dc.subjectcherry trees
dc.subjectPrunus avium
dc.subjectdiagnosis
dc.subjectCHLOROTIC RUSTY SPOT
dc.subjectCOMPLEX PATTERN
dc.titleDevelopment of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry diseaseen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionAgriculture, Food and Veterinary Sciences
dc.contributor.institutionCrop Protection and Climate Change
dc.contributor.institutionDepartment of Clinical, Pharmaceutical and Biological Science
dc.contributor.institutionCentre for Agriculture, Food and Environmental Management Research
dc.description.statusPeer reviewed
rioxxterms.versionofrecord10.1111/j.1365-3059.2007.01690.x
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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