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dc.contributor.authorChau, David Y.S.
dc.contributor.authorTint, Naing L.
dc.contributor.authorCollighan, Russell J.
dc.contributor.authorGriffin, Martin
dc.contributor.authorDua, Harminder S.
dc.contributor.authorShakesheff, Kevin M.
dc.contributor.authorRose, Felicity R. A. J.
dc.date.accessioned2013-12-03T16:00:01Z
dc.date.available2013-12-03T16:00:01Z
dc.date.issued2010-05
dc.identifier.citationChau , D Y S , Tint , N L , Collighan , R J , Griffin , M , Dua , H S , Shakesheff , K M & Rose , F R A J 2010 , ' The visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticles ' , British Journal of Ophthalmology , vol. 94 , no. 5 , pp. 648-653 . https://doi.org/10.1136/bjo.2009.163642
dc.identifier.issn0007-1161
dc.identifier.otherPURE: 2585523
dc.identifier.otherPURE UUID: c5ff5067-cbbe-42a8-91b8-de12fa427e47
dc.identifier.otherWOS: 000277374500026
dc.identifier.otherScopus: 77952140626
dc.identifier.urihttp://hdl.handle.net/2299/12257
dc.descriptionThis paper is freely available online under the BMJ Journals unlocked scheme, see http:// bjo.bmj.com/site/about/unlocked.xhtm
dc.description.abstractAims To demonstrate the potential use of in vitro poly (lactic-co-glycolic acid) (PLGA) microparticles in comparison with triamcinolone suspension to aid visualisation of vitreous during anterior and posterior vitrectomy. Methods PLGA microparticles (diameter 10-60 mu m) were fabricated using single and/or double emulsion technique(s) and used untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and compared with a standard triamcinolone suspension. The efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. Results Unmodified PLGA microparticles failed to adequately adhere to porcine vitreous and were readily washed out by irrigation. In contrast, modified transglutaminase-coated PLGA microparticles demonstrated a significant improvement in adhesiveness and were comparable to a triamcinolone suspension in their ability to enhance the visualisation of vitreous. This adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. Conclusion The use of transglutaminase-modified biodegradable PLGA microparticles represents a novel method of visualising vitreous and aiding vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension.en
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofBritish Journal of Ophthalmology
dc.subjectPREFERRED SUBSTRATE SEQUENCES
dc.subjectDISPLAYED PEPTIDE LIBRARY
dc.subjectTRIAMCINOLONE ACETONIDE
dc.subjectANTERIOR-CHAMBER
dc.subjectMICROBIAL TRANSGLUTAMINASE
dc.subjectTISSUE TRANSGLUTAMINASE
dc.subjectCATARACT-SURGERY
dc.subjectMICROSPHERES
dc.subjectCAPSULE
dc.subjectCELLS
dc.titleThe visualisation of vitreous using surface modified poly(lactic-co-glycolic acid) microparticlesen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Pharmacy
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1136/bjo.2009.163642
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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