dc.contributor.author | Bible, Ellen | |
dc.contributor.author | Chau, David Y.S. | |
dc.contributor.author | Alexander, Morgan R. | |
dc.contributor.author | Price, Jack | |
dc.contributor.author | Shakesheff, Kevin M. | |
dc.contributor.author | Modo, Michel | |
dc.date.accessioned | 2013-12-16T12:00:24Z | |
dc.date.available | 2013-12-16T12:00:24Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Bible , E , Chau , D Y S , Alexander , M R , Price , J , Shakesheff , K M & Modo , M 2009 , ' Attachment of stem cells to scaffold particles for intra-cerebral transplantation ' , Nature Protocols , vol. 4 , no. 10 , pp. 1440-1453 . https://doi.org/10.1038/nprot.2009.156 | |
dc.identifier.issn | 1754-2189 | |
dc.identifier.uri | http://hdl.handle.net/2299/12324 | |
dc.description.abstract | Cell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair. | en |
dc.format.extent | 14 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Protocols | |
dc.subject | IMPLANTATION SITE | |
dc.subject | STROKE DAMAGE | |
dc.subject | RECOVERY | |
dc.subject | GRAFTS | |
dc.subject | DIFFERENTIATION | |
dc.subject | RATS | |
dc.subject | LINE | |
dc.title | Attachment of stem cells to scaffold particles for intra-cerebral transplantation | en |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Health & Human Sciences Research Institute | |
dc.contributor.institution | Department of Pharmacy | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1038/nprot.2009.156 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |