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dc.contributor.authorBible, Ellen
dc.contributor.authorChau, David Y.S.
dc.contributor.authorAlexander, Morgan R.
dc.contributor.authorPrice, Jack
dc.contributor.authorShakesheff, Kevin M.
dc.contributor.authorModo, Michel
dc.date.accessioned2013-12-16T12:00:24Z
dc.date.available2013-12-16T12:00:24Z
dc.date.issued2009
dc.identifier.citationBible , E , Chau , D Y S , Alexander , M R , Price , J , Shakesheff , K M & Modo , M 2009 , ' Attachment of stem cells to scaffold particles for intra-cerebral transplantation ' , Nature Protocols , vol. 4 , no. 10 , pp. 1440-1453 . https://doi.org/10.1038/nprot.2009.156
dc.identifier.issn1754-2189
dc.identifier.otherPURE: 2585803
dc.identifier.otherPURE UUID: 4e13748c-4cc5-44ef-9ff8-137bcc8a983c
dc.identifier.otherWOS: 000275367400005
dc.identifier.otherScopus: 70349084163
dc.identifier.urihttp://hdl.handle.net/2299/12324
dc.description.abstractCell-replacement therapy and tissue regeneration using stem cells are of great interest to recover histological damage caused by neuro-degenerative disease or traumatic insults to the brain. To date, the main intra-cerebral delivery for these cells has been as a suspension in media through a thin needle. However, this does not provide cells with a support system that would allow tissue regeneration. Scaffold particles are needed to provide structural support to cells to form de novo tissue. In this 16-d protocol, we describe the generation and functionalization of poly (D,L-lactic-co-glycolic) acid (PLGA) particles to enhance cell attachment, the attachment procedure to avoid clumping and aggregation of cells and particles, and their preparation for intra-cerebral injection through a thin needle. Although the stem cell-scaffold transplantation is more complicated and labor-intensive than cell suspensions, it affords de novo tissue generation inside the brain and hence provides a significant step forward in traumatic brain repair.en
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofNature Protocols
dc.subjectIMPLANTATION SITE
dc.subjectSTROKE DAMAGE
dc.subjectRECOVERY
dc.subjectGRAFTS
dc.subjectDIFFERENTIATION
dc.subjectRATS
dc.subjectLINE
dc.titleAttachment of stem cells to scaffold particles for intra-cerebral transplantationen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionDepartment of Pharmacy
dc.description.statusPeer reviewed
rioxxterms.versionofrecordhttps://doi.org/10.1038/nprot.2009.156
rioxxterms.typeJournal Article/Review


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