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        Application of dried blood spots to determine vitamin D status in a large nutritional study with unsupervised sampling : the Food4Me project

        Author
        Hoeller, Ulrich
        Baur, Manuela
        Roos, Franz F
        Brennan, Lorraine
        Daniel, Hannelore
        Fallaize, Rosalind
        Forster, Hannah
        Gibney, Eileen R.
        Gibney, Mike
        Godlewska, Magdalena
        Hartwig, Kai
        Kolossa, Silvia
        Lambrinou, Christina P.
        Livingstone, Katherine M.
        Lovegrove, Julie A.
        Macready, Anna L.
        Manios, Yannis
        Marsaux, Cyril F M
        Martinez, J. Alfredo
        Celis-Morales, Carlos
        Moschonis, George
        Navas-Carretero, Santiago
        O'Donovan, Clare B.
        San-Cristobal, Rodrigo
        Saris, Wim H. M.
        Surwiłło, Agnieszka
        Traczyk, Iwona
        Tsirigoti, Lydia
        Walsh, Marianne C.
        Woolhead, Clara
        Mathers, John C.
        Weber, Peter
        Attention
        2299/17037
        Abstract
        An efficient and robust method to measure vitamin D (25-hydroxy vitamin D3 (25(OH)D3) and 25-hydroxy vitamin D2 in dried blood spots (DBS) has been developed and applied in the pan-European multi-centre, internet-based, personalised nutrition intervention study Food4Me. The method includes calibration with blood containing endogenous 25(OH)D3, spotted as DBS and corrected for haematocrit content. The methodology was validated following international standards. The performance characteristics did not reach those of the current gold standard liquid chromatography-MS/MS in plasma for all parameters, but were found to be very suitable for status-level determination under field conditions. DBS sample quality was very high, and 3778 measurements of 25(OH)D3 were obtained from 1465 participants. The study centre and the season within the study centre were very good predictors of 25(OH)D3 levels (P<0·001 for each case). Seasonal effects were modelled by fitting a sine function with a minimum 25(OH)D3 level on 20 January and a maximum on 21 July. The seasonal amplitude varied from centre to centre. The largest difference between winter and summer levels was found in Germany and the smallest in Poland. The model was cross-validated to determine the consistency of the predictions and the performance of the DBS method. The Pearson's correlation between the measured values and the predicted values was r 0·65, and the sd of their differences was 21·2 nmol/l. This includes the analytical variation and the biological variation within subjects. Overall, DBS obtained by unsupervised sampling of the participants at home was a viable methodology for obtaining vitamin D status information in a large nutritional study.
        Publication date
        2016-01
        Published in
        British Journal of Nutrition
        Published version
        https://doi.org/10.1017/S0007114515004298
        Other links
        http://hdl.handle.net/2299/17037
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