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        Automatic segmentation of odor maps in the mouse olfactory bulb using regularized non-negative matrix factorization

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        Author
        Soelter, Jan
        Schumacher, Jan
        Spors, Hartwig
        Schmuker, Michael
        Attention
        2299/19022
        Abstract
        Segmentation of functional parts in image series of functional activity is a common problem in neuroscience. Here we apply regularized non-negative matrix factorization (rNMF) to extract glomeruli in intrinsic optical signal (IOS) images of the olfactory bulb. Regularization allows us to incorporate prior knowledge about the spatio-temporal characteristics of glomerular signals. We demonstrate how to identify suitable regularization parameters on a surrogate dataset. With appropriate regularization segmentation by rNMF is more resilient to noise and requires fewer observations than conventional spatial independent component analysis (sICA). We validate our approach in experimental data using anatomical outlines of glomeruli obtained by 2-photon imaging of resting synapto-pHluorin fluorescence. Taken together, we show that rNMF provides a straightforward method for problem tailored source separation that enables reliable automatic segmentation of functional neural images, with particular benefit in situations with low signal-to-noise ratio as in IOS imaging.
        Publication date
        2014-09-01
        Published in
        Neuroimage
        Published version
        https://doi.org/10.1016/j.neuroimage.2014.04.041
        Other links
        http://hdl.handle.net/2299/19022
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