dc.contributor.author | Soelter, Jan | |
dc.contributor.author | Schumacher, Jan | |
dc.contributor.author | Spors, Hartwig | |
dc.contributor.author | Schmuker, Michael | |
dc.date.accessioned | 2017-07-20T16:05:03Z | |
dc.date.available | 2017-07-20T16:05:03Z | |
dc.date.issued | 2014-09-01 | |
dc.identifier.citation | Soelter , J , Schumacher , J , Spors , H & Schmuker , M 2014 , ' Automatic segmentation of odor maps in the mouse olfactory bulb using regularized non-negative matrix factorization ' , Neuroimage , vol. 98 , pp. 279-288 . https://doi.org/10.1016/j.neuroimage.2014.04.041 | |
dc.identifier.issn | 1053-8119 | |
dc.identifier.uri | http://hdl.handle.net/2299/19022 | |
dc.description | © 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/). | |
dc.description.abstract | Segmentation of functional parts in image series of functional activity is a common problem in neuroscience. Here we apply regularized non-negative matrix factorization (rNMF) to extract glomeruli in intrinsic optical signal (IOS) images of the olfactory bulb. Regularization allows us to incorporate prior knowledge about the spatio-temporal characteristics of glomerular signals. We demonstrate how to identify suitable regularization parameters on a surrogate dataset. With appropriate regularization segmentation by rNMF is more resilient to noise and requires fewer observations than conventional spatial independent component analysis (sICA). We validate our approach in experimental data using anatomical outlines of glomeruli obtained by 2-photon imaging of resting synapto-pHluorin fluorescence. Taken together, we show that rNMF provides a straightforward method for problem tailored source separation that enables reliable automatic segmentation of functional neural images, with particular benefit in situations with low signal-to-noise ratio as in IOS imaging. | en |
dc.format.extent | 10 | |
dc.format.extent | 2268587 | |
dc.language.iso | eng | |
dc.relation.ispartof | Neuroimage | |
dc.subject | Algorithms | |
dc.subject | Animals | |
dc.subject | Brain Mapping | |
dc.subject | Image Processing, Computer-Assisted | |
dc.subject | Mice | |
dc.subject | Odors | |
dc.subject | Olfactory Bulb | |
dc.subject | Optical Imaging | |
dc.subject | Smell | |
dc.title | Automatic segmentation of odor maps in the mouse olfactory bulb using regularized non-negative matrix factorization | en |
dc.contributor.institution | Department of Computer Science | |
dc.contributor.institution | School of Physics, Engineering & Computer Science | |
dc.contributor.institution | Centre of Data Innovation Research | |
dc.contributor.institution | Biocomputation Research Group | |
dc.description.status | Peer reviewed | |
rioxxterms.versionofrecord | 10.1016/j.neuroimage.2014.04.041 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |