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dc.contributor.authorGoh, Shan
dc.contributor.authorNgugi, Daniel
dc.contributor.authorLizundia, Regina
dc.contributor.authorHostettler, Isabel
dc.contributor.authorWoods, Kerry
dc.contributor.authorBallingall, Keith
dc.contributor.authorMacHugh, Niall D
dc.contributor.authorMorrison, W Ivan
dc.contributor.authorWeir, Willie
dc.contributor.authorShiels, Brian
dc.contributor.authorWerling, Dirk
dc.date.accessioned2018-02-16T16:17:49Z
dc.date.available2018-02-16T16:17:49Z
dc.date.issued2016-09-09
dc.identifier.citationGoh , S , Ngugi , D , Lizundia , R , Hostettler , I , Woods , K , Ballingall , K , MacHugh , N D , Morrison , W I , Weir , W , Shiels , B & Werling , D 2016 , ' Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells ' , PLoS ONE , vol. 11 , no. 9 , pp. e0162571 . https://doi.org/10.1371/journal.pone.0162571
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 11775189
dc.identifier.otherPURE UUID: 6d7d1692-3e37-4563-9cb2-bdc9302ce4e9
dc.identifier.otherPubMed: 27611868
dc.identifier.otherPubMedCentral: PMC5017765
dc.identifier.otherScopus: 84990929805
dc.identifier.otherORCID: /0000-0002-9028-0303/work/62751719
dc.identifier.urihttp://hdl.handle.net/2299/19784
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License, CC BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractAs part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.en
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.subjectJournal Article
dc.titleIdentification of Theileria lestoquardi Antigens Recognized by CD8+ T Cellsen
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionBiosciences Research Group
dc.description.statusPeer reviewed
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0162571
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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