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dc.contributor.authorTijerina-Rodrıguez, Laura
dc.contributor.authorVillarreal-Trevino, Licet
dc.contributor.authorBaines, Simon
dc.contributor.authorMorfin-Otero, Rayo
dc.contributor.authorCamacho-Ortiz, Adrian
dc.contributor.authorFlores-Trevino, Samantha
dc.contributor.authorMaldonado-Garza, Hector Jesus
dc.contributor.authorGarza-Gonzalez, Elvira
dc.contributor.authorRodriguez-Noriega, Eduardo
dc.contributor.authorRodrıguez-Noriega, Eduardo
dc.date.accessioned2019-08-07T01:40:25Z
dc.date.available2019-08-07T01:40:25Z
dc.date.issued2019-07-31
dc.identifier.citationTijerina-Rodrıguez , L , Villarreal-Trevino , L , Baines , S , Morfin-Otero , R , Camacho-Ortiz , A , Flores-Trevino , S , Maldonado-Garza , H J , Garza-Gonzalez , E , Rodriguez-Noriega , E & Rodrıguez-Noriega , E 2019 , ' High sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolates ' , PLoS ONE , vol. 14 , no. 7 , e0220671 , pp. e0220671 . https://doi.org/10.1371/journal.pone.0220671
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 17160763
dc.identifier.otherPURE UUID: fb5fa64a-7aac-4b17-8318-07cf47fec982
dc.identifier.otherScopus: 85070932649
dc.identifier.urihttp://hdl.handle.net/2299/21560
dc.description.abstractClostridium [Clostridioides] difficile infection (CDI) is one of the leading causes of diarrhea associated with medical care worldwide, and up to 60% of patients with CDI can develop a recurrent infection (R-CDI). A multi-species microbiota biofilm model of C. difficile was designed to evaluate the differences in the production of biofilms, sporulation, susceptibility to drugs, expression of sporulating (sigH, spo0A), quorum sensing (agrD1, and luxS), and adhesion-associated (slpA and cwp84) pathway genes between selected C. difficile isolates from R-CDI and non-recurrent patients (NR-CDI). We obtained 102 C. difficile isolates from 254 patients with confirmed CDI (66 from NR-CDI and 36 from R-CDI). Most of the isolates were biofilm producers, and most of the strains were ribotype 027 (81.374%, 83/102). Most C. difficile isolates were producers of biofilm (100/102), and most were strongly adherent. Sporulation was higher in the R-CDI than in the NR-CDI isolates (p = 0.015). The isolates from R-CDI patients more frequently demonstrated reduced susceptibility to vancomycin than isolates of NR-CDI patients (27.78% [10/36] and 9.09% [6/66], respectively, p = 0.013). The minimum inhibitory concentrations for vancomycin and linezolid against biofilms (BMIC) were up to 100 times and 20 times higher, respectively, than the corresponding planktonic MICs. Expression of sigH, spo0A, cwp84, and agrD1 was higher in R-CDI than in NR-CDI isolates. Most of the C. difficile isolates were producers of biofilms with no correlation with the ribotype. Sporulation was greater in R-CDI than in NR-CDI isolates in the biofilm model of C. difficile. The R-CDI isolates more frequently demonstrated reduced susceptibility to vancomycin and linezolid than the NR-CDI isolates in both planktonic cells and biofilm isolates. A higher expression of sporulating pathway (sigH, spo0A), quorum sensing (agrD1), and adhesion-associated (cwp84) genes was found in R-CDI than in NR-CDI isolates. All of these factors can have effect on the recurrence of the infection.en
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.subjectBiochemistry, Genetics and Molecular Biology(all)
dc.subjectAgricultural and Biological Sciences(all)
dc.titleHigh sporulation and overexpression of virulence factors in biofilms and reduced susceptibility to vancomycin and linezolid in recurrent Clostridium [Clostridioides] difficile infection isolatesen
dc.contributor.institutionDepartment of Biological and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionMicrobiology and Biotechnology
dc.contributor.institutionBiosciences Research Group
dc.contributor.institutionSchool of Life and Medical Sciences
dc.description.statusPeer reviewed
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85070932649&partnerID=8YFLogxK
rioxxterms.versionVoR
rioxxterms.versionofrecordhttps://doi.org/10.1371/journal.pone.0220671
rioxxterms.typeJournal Article/Review
herts.preservation.rarelyaccessedtrue


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