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        Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets

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        Author
        Antwi-Baffour, Samuel
        Malibha-Pinchbeck, Memory
        Stratton, Dan
        Jorfi, Samireh
        Lange, Sigrun
        Inal, Jameel
        Attention
        2299/22031
        Abstract
        This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (patient delay, PD), (iii) age and (iv) gender. In this cross-sectional study, n = 434 patients were analysed and Nanosight Tracking Analysis (NTA) used to quantify mEVs (vesicles of 150–500 nm diameter, isolated at 15,000 × g, β-tubulin-positive and staining for annexin V, but weak or negative for CD81). Overall plasma mEV levels (1.69 × 10 10 mEVs mL −1) were 2.3-fold higher than for uninfected controls (0.51 × 10 10 mEVs mL −1). Divided into four age groups, we found a bimodal distribution with 2.5- and 2.1-fold higher mEVs in infected children (<11 years old [yo]) (median:2.11 × 10 10 mEVs mL −1) and the elderly (>45 yo) (median:1.92 × 10 10 mEVs mL −1), respectively, compared to uninfected controls; parasite density varied similarly with age groups. There was a positive association between mEVs and parasite density (r = 0.587, p < 0.0001) and mEVs were strongly associated with PD (r = 0.919, p < 0.0001), but gender had no effect on plasma mEV levels (p = 0.667). Parasite density was also exponentially related to patient delay. Gender (p = 0.667) had no effect on plasma mEV levels. During periods of low parasitaemia (PD = 72h), mEVs were 0.93-fold greater than in uninfected controls. As 75% (49/65) of patients had low parasitaemia levels (20–500 parasites µL −1), close to the detection limits of microscopy of Giemsa-stained thick blood films (5–150 parasites µL −1), mEV quantification by NTA could potentially have early diagnostic value, and raises the potential of Pf markers in mEVs as early diagnostic targets.
        Publication date
        2019-12-18
        Published in
        Journal of Extracellular Vesicles
        Published version
        https://doi.org/10.1080/20013078.2019.1697124
        Other links
        http://hdl.handle.net/2299/22031
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