dc.contributor.author | Adrian Martinez-Melendez, Adrian | |
dc.contributor.author | Tijerina-Rodrıguez, Laura | |
dc.contributor.author | Morfin-Otero, Rayo | |
dc.contributor.author | Camacho-Ortiz, Adrian | |
dc.contributor.author | Villarreal-Trevino, Licet | |
dc.contributor.author | Sanchez-Alanıs, Hugo | |
dc.contributor.author | Rodrıguez-Noriega, Eduardo | |
dc.contributor.author | Baines, Simon | |
dc.contributor.author | Flores-Trevino, Samantha | |
dc.contributor.author | Maldonado-Garza, Hector Jesus | |
dc.contributor.author | Garza-Gonzalez, Elvira | |
dc.date.accessioned | 2020-03-26T01:05:56Z | |
dc.date.available | 2020-03-26T01:05:56Z | |
dc.date.issued | 2018-05-01 | |
dc.identifier.citation | Adrian Martinez-Melendez , A , Tijerina-Rodrıguez , L , Morfin-Otero , R , Camacho-Ortiz , A , Villarreal-Trevino , L , Sanchez-Alanıs , H , Rodrıguez-Noriega , E , Baines , S , Flores-Trevino , S , Maldonado-Garza , H J & Garza-Gonzalez , E 2018 , ' Circulation of Highly Drug-Resistant Clostridium difficile Ribotypes 027 and 001 in Two Tertiary-Care Hospitals in Mexico ' , Microbial Drug Resistance , vol. 24 , no. 4 , pp. 386-392 . https://doi.org/10.1089/mdr.2017.0323 | |
dc.identifier.issn | 1931-8448 | |
dc.identifier.uri | http://hdl.handle.net/2299/22495 | |
dc.description | © 2018, Mary Ann Liebert, Inc. | |
dc.description.abstract | OBJECTIVE: To assess drug susceptibility and characterize Clostridium difficile ribotypes in isolates from two tertiary-care hospitals in Mexico. METHODS: Isolates were evaluated for genotyping, antimicrobial susceptibility testing and detection of mutations associated with drug resistance. PCR ribotyping was performed using a combination of gel-based and capillary electrophoresis-based approaches. RESULTS: MIC50 and MIC90 were ≥128 mg/L for ciprofloxacin, erythromycin, clindamycin, and rifampicin. There was no reduced susceptibility to metronidazole or tetracycline; however, reduced susceptibility to vancomycin (≥4 mg/L) and fidaxomicin (≥2 mg/L) was detected in 50 (40.3%) and 4 (3.2%) isolates, respectively. Furthermore, the rpoB Arg505Lys mutation was more frequently detected in isolates with high minimum inhibitory concentration (MIC) to rifampicin (≥32 mg/L) (OR = 52.5; 95% CI = 5.17-532.6; p < 0.000). Of the 124 C. difficile isolates recovered, 84 (66.7%) were of ribotype 027, 18 (14.5%) of ribotype 001, and the remainder were other ribotypes (353, 255, 220, 208, 176, 106, 076, 020, 019, 017, 014, 012, 003, and 002). CONCLUSION: Ribotypes 027 and 001 were the most frequent C. difficile isolates recovered in this study, and demonstrated higher MICs. Furthermore, we found four isolates with reduced susceptibility to fidaxomicin, raising a concern since this drug is currently unavailable in Mexican Hospitals. | en |
dc.format.extent | 7 | |
dc.format.extent | 399217 | |
dc.language.iso | eng | |
dc.relation.ispartof | Microbial Drug Resistance | |
dc.subject | Clostridium difficile | |
dc.subject | drug resistance | |
dc.subject | fidaxomicin | |
dc.subject | ribotype 001 | |
dc.subject | ribotypes | |
dc.subject | Microbiology | |
dc.subject | Immunology | |
dc.subject | Pharmacology | |
dc.subject | Microbiology (medical) | |
dc.title | Circulation of Highly Drug-Resistant Clostridium difficile Ribotypes 027 and 001 in Two Tertiary-Care Hospitals in Mexico | en |
dc.contributor.institution | Microbiology and Biotechnology | |
dc.contributor.institution | School of Life and Medical Sciences | |
dc.contributor.institution | Biosciences Research Group | |
dc.contributor.institution | Centre for Research in Mechanisms of Disease and Drug Discovery | |
dc.contributor.institution | Department of Clinical, Pharmaceutical and Biological Science | |
dc.contributor.institution | Centre for Future Societies Research | |
dc.description.status | Peer reviewed | |
dc.date.embargoedUntil | 2019-05-01 | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85054460749&partnerID=8YFLogxK | |
rioxxterms.versionofrecord | 10.1089/mdr.2017.0323 | |
rioxxterms.type | Journal Article/Review | |
herts.preservation.rarelyaccessed | true | |