Browsing Research publications by Author "Stratton, Dan"
Now showing items 1-8 of 8
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Characterisation of microvesicles released from cells constitutively and upon stimulation
Stratton, Dan; Inal, Jameel (2012-09-13)Constitutively released microvesicles (cMVs) are released as a part of normal cell physiology and this is summarised in Fig. 1 (1). However stimulated microvesicles (sMVs) are released as a result of a number of possible ... -
A Coxsackievirus B1-mediated nonlytic Extracellular Vesicle-to-cell mechanism of virus transmission and its possible control through modulation of EV release
Jorfi, Samireh; Ansa-Addo, Ephraim Abrokwa; Mariniello, Katia; Warde, Purva; Bin Senian, Ahmad Asyraf; Stratton, Dan; Bax, Bridget E; Levene, Michelle; Lange, Sigrun; Inal, Jameel Malhador (2023-09-04)Like most non-enveloped viruses, CVB1 mainly uses cell lysis to spread. Details of a nonlytic virus transmission remain unclear. Extracellular Vesicles (EVs) transfer biomolecules between cells. We show that CVB1 entry ... -
Inhibition of microvesiculation sensitizes prostate cancer cells to chemotherapy and reduces docetaxel dose required to limit tumor growth in vivo
Jorfi, Samireh; Ansa-Addo, Ephraim A.; Kholia, Sharad; Stratton, Dan; Valley, Shaunelle; Lange, Sigrun; Inal, Jameel (2015-08-25)Microvesicles shed from cells carry constituents of the cell cytoplasm, including, of importance in multidrug resistance to cancer chemotherapy, drugs that the tumor cell attempts to efflux. To see whether such drugs could ... -
Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
De Sousa, Karina P.; Rossi, Izadora; Abdullahi, Mahamed; Ramirez, Marcel Ivan; Stratton, Dan; Inal, Jameel Malhador (2023-01-13)Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and ... -
Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance
Stratton, Dan; Lange, Sigrun; Kholia, Sharad; Jorfi, Samireh; Antwi-Baffour, Samuel; Inal, Jameel (2014-10-24)Using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D (label-free system) measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, we showed the attachment, over a 60 min ... -
Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9
Stratton, Dan; Moore, Colin; Antwi-Baffour, Samuel; Lange, Sigrun; Inal, Jameel (2015-05-08)We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, ... -
Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets
Antwi-Baffour, Samuel; Malibha-Pinchbeck, Memory; Stratton, Dan; Jorfi, Samireh; Lange, Sigrun; Inal, Jameel (2019-12-18)This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, ... -
Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles
Stratton, Dan; Moore, Colin; Zheng, Lei; Lange, Sigrun; Inal, Jameel (2015-05-08)MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, ...