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dc.contributor.authorTheophilus, B.D.M.
dc.contributor.authorRapley, Ralph
dc.date.accessioned2011-08-15T14:01:19Z
dc.date.available2011-08-15T14:01:19Z
dc.date.issued2010
dc.identifier.citationTheophilus , B D M & Rapley , R 2010 , PCR mutation detection protocols . 2nd edn , Humana Press .
dc.identifier.isbn978-1-60761-946-8
dc.identifier.otherPURE: 305359
dc.identifier.otherPURE UUID: 8b121e29-0032-4ff1-868a-86c90559db7f
dc.identifier.urihttp://hdl.handle.net/2299/6224
dc.descriptionTheophilus, Bimal D.M., Rapley, Ralph (Eds.), PCR Mutation Detection Protocols, 2nd Edition, Humana Press. eBOOK ISBN: 978-1-60761-947-5, Hardcover ISBN: 978-1-60761-946-8, DOI: 10.1007/978-1-60761-947-5. © 2011 Springer Science + Business Media, LLC
dc.description.abstractThis is the second edition of a text that was followed by the huge growth in DNA sequence information, the availability of bioinformatics and mutation databases, and the resources to detect and confirm mutations. This all secured the place of molecular diagnostics in medicine as well as mutation detection by the polymerase chain reaction (PCR). In this text researchers of the field renew this subject to encompass contemporary information and key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The book further explores core topics such as Southern blotting, accurate diagnostics with high throughput, and microarray systems. The book is composed in the popular series format of Methods in Molecular Biology. Its chapters include brief introductions of the topics, lists of the materials and reagents that are essential, list-form easily to follow laboratory protocols, and notes which provide often hidden information that is necessary to implement the method successfully. This text serves to help postgraduate scientists, researchers, and clinicians who are already occupied in the field as well as supplying a solid first step to those who are new to the notion of employing this method in the laboratory.en
dc.format.extent305
dc.language.isoeng
dc.publisherHumana Press
dc.titlePCR mutation detection protocolsen
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionHealth & Human Sciences Research Institute
rioxxterms.typeBook
herts.preservation.rarelyaccessedtrue


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