Effects of nano CuO on hippocampal neurons and differentiated PC12 cells
The effects of nanoparticles of CuO on delayed rectifier potassium current (Ik) were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus by using the whole-cell patch-clamp techniques and the effects on differentiated PC12 cell viability were studied with MTT assay. Nanoparticles of CuO mainly inhibited Ik in the concentration of 5×10－5 g/mL. Nanoparticles of CuO didn't shift the steady-state activation curve of Ik but negatively shift the inactivation curve of Ik. Nanoparticles of CuO (5×10－4, 5×10－5 and 5×10－6 g/mL) induced differentiated PC12 cells damage with dose-and time-dependent manner.