Design, Synthesis and Antimicrobial Evaluation of the in vitro Efficacy of Novel Diketones

A series of novel diketones were designed and synthesised in an attempt to develop potent antimicrobial agents. Their structures were confirmed by spectroscopic techniques i.e. 1H and 13C-NMR, IR and LC-MS. The minimum inhibitory concentration (MIC) was determined against a wide range of bacteria using two fold micro broth dilution method. Among the test Compounds DK2, DK6, DK7, DK11, DK12, DK16 and DK17 were found to have excellent activity against all Gram positive bacteria with MIC values ranging 16-0.1μg/ml. The in vitro cytotoxity of all the active compounds was assessed by MTT clorimetric assay. The bioactive compounds exhibited IC50 values against Caco-2 cells rainging 64-8ug/ml