- UHRA Home
- Browsing by Author
Browsing by Author "Buck, K.W."
Now showing items 1-16 of 16
-
The behaviour of tomato golden mosaic virus DNA in cultured cells isolated from systemically infected tobacco leaves
Slomka, M.J.; Buck, K.W.; Coutts, Robert H.A. (1989-03)When callus tissue was cultured from leaf pieces taken from a Nicotiana tabacum cv. Xanthi nc. plant systemically infected with tomato golden mosaic virus (TGMV), TGMV-specific DNA persisted for up to 6 months in culture. ... -
Cassava latent virus specific DNAs in mosaic diseased cassava of Nigerian origin
Coutts, Robert H.A.; Buck, K.W. (1987-09-01)Het genoom van het 'cassava latent' virus (CLV), een geminivirus, bestaat uit twee cirkelvormige, enkelstrengige DNA-moleculen nl. DNA 1 (2,78 kb) en DNA 2 (2,72 kb). DNA, verkregen uit Nicotiana benthamiana-planten, die ... -
Characterisation of multimeric DNA forms associated with tomato golden mosaic virus infection
Slomka, M.J.; Buck, K.W.; Coutts, Robert H.A. (1988-03-01)Homodimeric and trimeric double-stranded DNA forms of both components of the genome of the geminivirus, tomato golden mosaic virus have been isolated from infected Nicotiana tabacum plants and characterised. -
Characterisation of tomato golden mosaic virus as a geminivirus
Hamilton, W.D.O.; Sanders, R.C.; Coutts, Robert H.A.; Buck, K.W. (1981-08) -
Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components
Hamilton, W.D.O.; Bisaro, D.M.; Coutts, Robert H.A.; Buck, K.W. (1983-11-11)Linear double-stranded (ds)DNA, obtained by excision of the cloned A and B components of tomato golden mosaic virus (TGMV) from recombinant plasmids, was found to infect plants and to elicit symptoms identical to those ... -
DNA and RNA polymerase activities of nuclei and hypotonic extracts of nuclei isolated from tomato golden mosaic virus infected tobacco leaves
Coutts, Robert H.A.; Buck, K.W. (1985-11-11)Nuclei and hypotonically leached extracts of nuclei prepared from tomato golden mosaic virus (TGMV)-infected Nicotiana benthamiana leaves have been used in in vitro DNA and RNA polymerisation reactions. The synthesis of ... -
Gene amplification and expression in plants by a replicating geminivirus vector
Hayes, R.J.; Petty, I.T.D.; Coutts, Robert H.A.; Buck, K.W. (1988-01-01)The use of plant DNA viruses as vectors for the transfer of foreign genes to plants offers two potential advantages over other, existing methods of producing transgenic plants. First, these viruses sys-temically infect ... -
Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus
Macdowell, S.W.; Coutts, Robert H.A.; Buck, K.W. (1986-10-01)A subgenomic single-stranded DNA present in particles of the geminivirus, tomato golden mosaic virus, has been shown by electron microscope heteroduplex mapping and Southern hybridisation analysis to consist of circular ... -
Molecular characterization of a partitivirus from Ophiostoma himal-ulmi
Crawford, L.J.; Osman, T.A.M.; Coutts, Robert H.A.; Buck, K.W.; Booy, F.P.; Brasier, C.M. (2006-08)The complete nucleotide sequences of two double-stranded (ds) RNA molecules, S1 (1,744 bp) and S2 (1,567 bp), isolated from an isolate HP62 of the Himalayan Dutch elm disease fungus, Ophiostoma himal-ulmi, were determined. ... -
Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus
Bisaro, D.M.; Hamilton, W.D.O.; Coutts, Robert H.A.; Buck, K.W. (1982-08-25)We report the molecular cloning of the tomato golden mosaic virus (TGMV) genome in the E. coli plasmid pAT 153. The results of this work conclusively show that TGMV DNA consists of two components (designated A and B) of ... -
Negatively supercoiled DNA from plants infected with a single-stranded DNA virus
Sunter, G.; Coutts, Robert H.A.; Buck, K.W. (1984-02-14)A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled ... -
The nucleotide sequence of cloned wheat dwarf virus DNA
MacDowell, S.W.; Macdonald, H.; Hamilton, W.D.; Coutts, Robert H.A.; Buck, K.W. (1985-09)Restriction analysis and cloning of virus-specific double-stranded DNA isolated from plants infected with wheat dwarf virus (WDV) indicated that the virus genome, like that of maize streak virus (MSV), consists of a single ... -
S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B : Identification of regions of potential alternative secondary structure and regulatory function
Sunter, G.; Buck, K.W.; Coutts, Robert H.A. (1985-07-11)The sensitivity of the supercoiled double-stranded form of the DNA of tanato golden mosaic virus (TGMV), a geminivirus, to the single-strand specific enzyme S1 nuclease has been demonstrated. Specific S1 cleavage sites ... -
Sequence of RNA-dependent RNA polymerase genes provides evidence for three more distinct mitoviruses in Ophiostoma novo-ulmi isolate Ld
Doherty, M.; Coutts, Robert H.A.; Brasier, C.M.; Buck, K.W. (2006-08-01)Three of the twelve double-stranded (ds) RNAs, dsRNAs 1a, 1b and 3b, which are located in the mitochondria of a diseased isolate, Ld, of the Dutch elm disease fungus, Ophiostoma novo-ulmi have been cDNA cloned and sequenced. ... -
Stability and expression of bacterial genes in replicating geminivirus vectors in plants
Hayes, R.J.; Coutts, Robert H.A.; Buck, K.W. (1989-04-11)Bacterial beta-glucuronidase (gus) and neomycin phosphotransferase (neo) genes were introduced into coat protein replacement vectors based on DNA A of tomato golden mosaic virus (TGMV). Recombinant gus and neo vectors up ... -
Systemic infection of petunia by mechanical inoculation with tomato golden mosaic virus
Petty, I.T.D.; Buck, K.W.; Coutts, Robert H.A. (1988)Aangetoond werd dat Petunia hybrida systemisch kan worden geïnfecteerd met het 'tomato golden mosaic virus' (TGMV), een virus dat behoort tot de groep van de geminivirussen. Mechanische inoculatie van petuniaplanten met ...