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Antisense PNA accumulates in Escherichia coli and mediates a long post-antibiotic effect
Nikravesh, Abbas; Dryselius, Rikard; Faridani, Omid R; Goh, Shan; Sadeghizadeh, Majid; Behmanesh, Mehrdad; Ganyu, Anita; Klok, Erik Jan; Zain, Rula; Good, Liam (2007-08-01)Antisense agents that target growth-essential genes display surprisingly potent bactericidal properties. In particular, peptide nucleic acid (PNA) and phosphorodiamidate morpholino oligomers linked to cationic carrier ... -
Comparison of pediatric and adult antibiotic-associated diarrhea and Clostridium difficile infections
McFarland, Lynne Vernice; Ozen, Metehan; Dinleyici, Ener Cagri; Goh, Shan (2016-03-21)Antibiotic-associated diarrhea (AAD) and Clostridium difficile infections (CDI) have been well studied for adult cases, but not as well in the pediatric population. Whether the disease process or response to treatments ... -
Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli
Goh, Shan; Boberek, Jaroslaw M; Nakashima, Nobutaka; Stach, Jem; Good, Liam (2009-06-26)BACKGROUND: Genes essential for bacterial growth are of particular scientific interest. Many putative essential genes have been identified or predicted in several species, however, little is known about gene expression ... -
Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle
Goh, Shan; Kolakowski, Jeannine; Holder, Angela; Pfuhl, Mark; Ngugi, Daniel; Ballingall, Keith; Tombacz, Kata; Werling, Dirk (2021-07-08)East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every ... -
The development of species-specific antisense peptide nucleic acid method for the treatment and detection of viable Salmonella
Adebowale, Oluwawemimo; Goh, Shan; Good, Liam (2020-06)Genotypic based detection methods using specific target sites in the pathogen genome can complement phenotypic identification. We report the development of species-specific antisense peptide nucleic acid (PNA) combined ... -
Editorial: Microbial interactions of Clostridioides difficile
Goh, Shan; Mullany, Peter; Riley, Thomas V. (2023-01-06) -
Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells
Goh, Shan; Ngugi, Daniel; Lizundia, Regina; Hostettler, Isabel; Woods, Kerry; Ballingall, Keith; MacHugh, Niall D; Morrison, W Ivan; Weir, Willie; Shiels, Brian; Werling, Dirk (2016-09-09)As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata ... -
A laboratory-based evaluation of tube blocking and microbial risks associated with one blended enteral feed recipe
Madden, Angela; Baines, Simon; Bothwell, Simone; Chen, Elise; Goh, Shan; Jerome, Lee; Sommariva-Nagle, Cara; Szychta, Malgorzata (2019-10-01)Background: Concerns associated with blended enteral feeds include the risk of blocked tubes and microbial contamination, although the available evidence is limited. The present laboratory-based investigation aimed to ... -
Membrane vesicles of Clostridioides difficile and other Clostridial species
Goh, Shan; Inal, Jameel (Springer Nature, 2024-01-05)Membrane vesicles are secreted by growing bacterial cells and are important components of the bacterial secretome, with a role in delivering effector molecules that ultimately enable bacterial survival. Membrane vesicles ... -
Molecular basis for DarT ADP-ribosylation of a DNA base
Schuller, Marion; Butler, Rachel; Ariza, Antonio; Tromans-Coia, Callum; Jankevicius, Gytis; Claridge, Tim; Kendall, Sharon; Goh, Shan; Stewart, Graham; Ahel, Ivan (2021-08-26)ADP-ribosyltransferases use NAD+ to catalyse substrate ADP-ribosylation1, and thereby regulate cellular pathways or contribute to toxin-mediated pathogenicity of bacteria2–4. Reversible ADP-ribosylation has traditionally ... -
The Novel Phages phiCD5763 and phiCD2955 Represent Two Groups of Big Plasmidial Siphoviridae Phages of Clostridium difficile
Ramírez-Vargas, Gabriel; Goh, Shan; Rodríguez, César (2018-01-22)Until recently, Clostridium difficile phages were limited to Myoviruses and Siphoviruses of medium genome length (32–57 kb). Here we report the finding of phiCD5763, a Siphovirus with a large extrachromosomal circular ... -
Oxacillin sensitization of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro
Goh, Shan; Loeffler, Anette; Lloyd, David H; Nair, Sean P; Good, Liam (2015-11-11)BACKGROUND: Antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. Antisense inhibitors can be gene and pathogen ... -
Phage ϕC2 mediates transduction of Tn6215, encoding erythromycin resistance, between Clostridium difficile strains
Goh, Shan; Hussain, Haitham; Chang, Barbara J; Emmett, Warren; Riley, Thomas V; Mullany, Peter (2013-11-19)UNLABELLED: In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(-6) per PFU. The transductants were ... -
Re-sensitization of Mycobacterium smegmatis to Rifampicin Using CRISPR Interference Demonstrates Its Utility for the Study of Non-essential Drug Resistance Traits
Faulkner, Valwynne; Cox, Adrienne; Goh, Shan; van Bohemen, Annelies; Gibson, Amanda; Liebster, Oliver; Wren, Brendan; Willcocks, Sam; Kendall, Sharon (2021-02-01)A greater understanding of the genes involved in antibiotic resistance in Mycobacterium tuberculosis (Mtb) is necessary for the design of improved therapies. Clustered regularly interspaced short palindromic repeat ... -
Removal of mobile genetic elements from the genome of Clostridioides difficile and the implications for the organism’s biology
Hussain, Haitham; Nubgan, Amer; Rodríguez, César; Imwattana, Korakrit; Knight, Daniel; Parthala, Valerija; Mullany, Peter; Goh, Shan (2024-06-20)Clostridioides difficile is an emerging pathogen of One Health significance. Its highly variable genome contains mobile genetic elements (MGEs) such as transposons and prophages that influence its biology. Systematic ... -
Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli
Goh, Shan; Hohmeier, Angela; Stone, Timothy C; Offord, Victoria; Sarabia, Francisco; Garcia-Ruiz, Cristina; Good, Liam (2015-08-15)Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes ... -
Species-selective killing of bacteria by antimicrobial peptide-PNAs
Mondhe, Madhav; Chessher, Ashley; Goh, Shan; Good, Liam; Stach, James E M (2014-02-18)Broad-spectrum antimicrobials kill indiscriminately, a property that can lead to negative clinical consequences and an increase in the incidence of resistance. Species-specific antimicrobials that could selectively kill ... -
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
Uguru, Gabriel C; Mondhe, Madhav; Goh, Shan; Hesketh, Andrew; Bibb, Mervyn J; Good, Liam; Stach, James E M (2013-06-27)We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic ...