- UHRA Home
- Browsing by Author
Browsing by Author "Lange, Sigrun"
Now showing items 1-20 of 20
-
Animal Models of Human Disease
Lange, Sigrun; Inal, Jameel (2023-10-31)The use of animal models of human disease is critical for furthering our understanding of disease mechanisms, for the discovery of novel targets for treatment, and for translational research. This Special Topic entitled ... -
Cannabidiol (CBD) is a novel inhibitor for exosome and microvesicle (EMV) release in cancer
Kosgodage, Uchini S; Mould, Rhys; Henley, Aine; Nunn, alistair; Guy, Geoffrey; Thomas, E. Louise; Inal, Jameel; Bell, Jimmy; Lange, Sigrun (2018-08-13)Exosomes and microvesicles (EMV) are lipid bilayer-enclosed structures, released by cells and involved in intercellular communication through transfer of proteins and genetic material. EMV release is also associated with ... -
Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells
Kosgodage, Uchini S; Uysal-Onganer, Pinar; MacLatchy, Amy; Mould, Rhys; Nunn, Alistair; Guy, Geoffrey; Kraev, Igor; Chatterton, Nicholas; Inal, Jameel; Thomas, E. Louise; Bell, Jimmy; Lange, Sigrun (2019-03-01)Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer ... -
Cannabidiol Is a Novel Modulator of Bacterial Membrane Vesicles
Kosgodage, Uchini S; Matewele, Paul; Awamaria, Brigitte; Kraev, Igor; Warde, Purva; Mastroianni, Giulia; Nunn, alistair; Guy, Geoffrey; Bell, Jimmy; Inal, Jameel; Lange, Sigrun (2019-09-10)Membrane vesicles (MVs) released from bacteria participate in cell communication and host-pathogen interactions. Roles for MVs in antibiotic resistance are gaining increased attention and in this study we investigated if ... -
Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy
Kosgodage, Uchini S; Trindade, Rita P; Thompson, Paul R; Inal, Jameel M; Lange, Sigrun (2017-05-09)Microvesicle (MV) release from tumour cells influences drug retention, contributing to cancer drug resistance. Strategically regulating MV release may increase drug retention within cancer cells and allow for lower doses ... -
A Coxsackievirus B1-mediated nonlytic Extracellular Vesicle-to-cell mechanism of virus transmission and its possible control through modulation of EV release
Jorfi, Samireh; Ansa-Addo, Ephraim Abrokwa; Mariniello, Katia; Warde, Purva; Bin Senian, Ahmad Asyraf; Stratton, Dan; Bax, Bridget E; Levene, Michelle; Lange, Sigrun; Inal, Jameel Malhador (2023-09-04)Like most non-enveloped viruses, CVB1 mainly uses cell lysis to spread. Details of a nonlytic virus transmission remain unclear. Extracellular Vesicles (EVs) transfer biomolecules between cells. We show that CVB1 entry ... -
The emerging role of exosome and microvesicle- (EMV-) based cancer therapeutics and immunotherapy
Moore, Colin; Kosgodage, Uchini; Lange, Sigrun; Inal, Jameel M (2017-08-01)There is an urgent need to develop new combination therapies beyond existing surgery, radio- and chemo-therapy, perhaps initially combining chemotherapy with the targeting specificities of immunotherapy. For this, strategies ... -
Inhibition of microvesiculation sensitizes prostate cancer cells to chemotherapy and reduces docetaxel dose required to limit tumor growth in vivo
Jorfi, Samireh; Ansa-Addo, Ephraim A.; Kholia, Sharad; Stratton, Dan; Valley, Shaunelle; Lange, Sigrun; Inal, Jameel (2015-08-25)Microvesicles shed from cells carry constituents of the cell cytoplasm, including, of importance in multidrug resistance to cancer chemotherapy, drugs that the tumor cell attempts to efflux. To see whether such drugs could ... -
Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance
Stratton, Dan; Lange, Sigrun; Kholia, Sharad; Jorfi, Samireh; Antwi-Baffour, Samuel; Inal, Jameel (2014-10-24)Using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D (label-free system) measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, we showed the attachment, over a 60 min ... -
Mesenchymal stromal cell derived extracellular vesicles reduce hypoxia-ischaemia induced perinatal injury
Sisa, Claudia; Kholia, Sharad; Naylor, Jordan; Herrera Sanchez, Maria Beatriz; Bruno, Stefania; Deregibus, Maria Chaiara; Camussi, Giovanni; Inal, Jameel; Lange, Sigrun; Hristova, Mariya (2019-03-19)Background: Neonatal hypoxic-ischemic (HI) insult is a leading cause of disability and death in newborns, with therapeutic hypothermia being the only currently available clinical intervention. Thus there is a great need ... -
Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9
Stratton, Dan; Moore, Colin; Antwi-Baffour, Samuel; Lange, Sigrun; Inal, Jameel (2015-05-08)We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, ... -
A novel role for peptidylarginine deiminases in microvesicle release reveals therapeutic potential of PAD inhibition in sensitizing prostate cancer cells to chemotherapy
Kholia, Sharad; Jorfi, Samireh; Thompson, Paul R.; Causey, Corey P.; Nicholas, Anthony P.; Inal, Jameel M.; Lange, Sigrun (2015-06-22)Introduction: Protein deimination, defined as the post-translational conversion of protein-bound arginine to citrulline, is carried out by a family of 5 calcium-dependent enzymes, the peptidylarginine deiminases (PADs) and ... -
Peptidylarginine deiminase inhibitors reduce bacterial membrane vesicle release and sensitize bacteria to antibiotic treatment
Kosgodage, Uchini S; Matewele, Paul; Mastroianni, Giulia; Lange, Sigrun; Kraev, Igor; Brotherton, Dominik; Awamaria, Brigitte; Nicholas, Anthony P.; Inal, Jameel (2019-06-27)Outer membrane and membrane vesicles (OMV/MV) are released from bacteria and participate in cell communication, biofilm formation and host-pathogen interactions. Peptidylarginine deiminases (PADs) are phylogenetically ... -
Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines
Uysal-Onganer, Pinar; MacLatchy, Amy; Mahmoud, Rayan; Kraev, Igor; Thompson, Paul R; Inal, Jameel; Lange, Sigrun (2020-02-22)Glioblastoma multiforme (GBM) is an aggressive adult brain tumour with poor prognosis. Roles for peptidylarginine deiminases (PADs) in GBM have recently been highlighted. Here, two GBM cell lines were treated with PAD2, ... -
Peptidylarginine Deiminases Post-Translationally Deiminate Prohibitin and Modulate Extracellular Vesicle Release and MicroRNAs in Glioblastoma Multiforme
Kosgodage, Uchini S; Uysal-Onganer, Pinar; MacLatchy, Amy; Kraev, Igor; Chatterton, Nicholas; Nicholas, Anthony P.; Inal, Jameel; Lange, Sigrun (2018-12-28)Glioblastoma multiforme (GBM) is the most aggressive form of adult primary malignant brain tumour with poor prognosis. Extracellular vesicles (EVs) are a key-mediator through which GBM cells promote a pro-oncogenic ... -
Peptidylarginine Deiminases-Roles in Cancer and Neurodegeneration and Possible Avenues for Therapeutic Intervention via Modulation of Exosome and Microvesicle (EMV) Release?
Lange, Sigrun; Gallagher, Mark; Kholia, Sharad; Kosgodage, Uchini S; Hristova, Mariya; Hardy, John; Inal, Jameel M (2017-06-05)Exosomes and microvesicles (EMVs) are lipid bilayer-enclosed structures released from cells and participate in cell-to-cell communication via transport of biological molecules. EMVs play important roles in various pathologies, ... -
A Pilot Study on Peptidylarginine Deiminases and Protein Deimination in Animal Cancers across Vertebrate Species
Inal, Jameel M.; Hristova, Mariya; Lange, Sigrun (2022-08-04)PADs are a group of calcium-dependent enzymes that play key roles in inflammatory pathologies and have diverse roles in cancers. PADs cause irreversible post-translational modification of arginine to citrulline, leading ... -
Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets
Antwi-Baffour, Samuel; Malibha-Pinchbeck, Memory; Stratton, Dan; Jorfi, Samireh; Lange, Sigrun; Inal, Jameel (2019-12-18)This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, ... -
Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles
Stratton, Dan; Moore, Colin; Zheng, Lei; Lange, Sigrun; Inal, Jameel (2015-05-08)MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, ... -
Treatment of Prostate Cancer Using Deimination Antagonists and Microvesicle Technology.
Lange, Sigrun; Kholia, Sharad; Kosgodage, Uchini S; Inal, Jameel (Springer Nature, 2017)Cellular microvesicle (MV) release, which occurs in most eukaryotic cells, is also involved in cancer progression and has recently been associated with peptidylarginine deiminase (PAD)-driven protein deimination. Evidence ...