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Browsing by Author "MacKenzie, Louise Susan"
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COX isoforms in aortic endothelial cells from young adult and mature adult mice
Lundberg, M.H.; MacKenzie, Louise Susan; Vojnovic, Ivana; Warner, Timothy D.; Mitchell, Jane A. (2009) -
The ‘EDHF’ Antagonist 14, 15 Epoxyeicosa-5(Z)-Enoic Acid has Vasodilator Properties in Mesenteric Vessels
MacKenzie, Louise Susan; Falck, John R.; Mitchell, Jane A. (2004-07)There is now overwhelming evidence for Epoxyeicosatrienoic acids (EETs) as endothelial derived hyperpolarising factor (EDHF). Most recently, a number of pharmacological tools have been developed for the study of EETs in ... -
Effect of COX-1 or COX-2 gene deletion on prostacyclin formation in the heart
Leadbeater, Philip DM; Vojnovic, Ivana; MacKenzie, Louise Susan; Lundberg, Martina; Gashaw, Hime; Paul-Clark, Mark; Warner, Timothy D.; Mitchell, Jane (2009) -
Effects of Apamin and Charybdtoxin on Endothelium Independent Vasodilatation : Implications in the study of EDHF
MacKenzie, Louise Susan; Carrier, M.J.; Mitchell, J.A. (2005-12)Endothelial derived hyperpolarizing factor (EDHF), together with endothelial derived NO and prostacyclin represent the major endogenous vasodilator hormone pathways (Coleman et al, 2004). Since the chemical nature of EDHF ... -
Evidence that Diclofenac and Celecoxib are thyroid hormone receptor beta antagonists
Zloh, Mire; Perez Diaz, Noelia; Tang, Leslie; Patel, Pryank; MacKenzie, Louise Susan (2016-02-01)Long term use of NSAIDs is linked to side effects such as gastric bleeding and myocardial infarction. AIMS: Use of in silico methods and pharmacology to investigate the potential for NSAIDs diclofenac, celecoxib and naproxen ... -
Harnessing the benefits of PPARβ/δ agonists
MacKenzie, Louise Susan; Lione, Lisa (2013-12)Lipid mediators have complex effects on the cell; one of the key transcriptional factors that moderate proliferation and inflammatory effects is PPARβ/δ. Following highly successful clinical trials using the PPARβ/δ agonists ... -
In silico modelling of prostacyclin and other lipid mediators to nuclear receptors reveal novel thyroid hormone beta receptor antagonist properties.
Perez Diaz, Noelia; Zloh, Mire; Patel, Pryank; MacKenzie, Louise Susan (2016-01-01)Prostacyclin (PGI2) is a key mediator involved in cardiovascular homeostasis, acting predominantly on two receptor types; cell surface IP receptor and cytosolic peroxisome proliferator activated receptor (PPAR) β/δ. Having ... -
Linking Induction and Transrepression of PPARβ/δ with Cellular Function
Perez Diaz, Noelia; MacKenzie, Louise Susan (2015-01-26)Peroxisome proliferator activated receptors (PPARs) are ligand-activated transcription factors and members of the nuclear hormone receptor superfamily. PPARβ/δ is ubiquitously expressed and has a central role in homeostasis, ... -
Linking Phospholipase C isoforms with differentiation function in human vascular smooth muscle cells
MacKenzie, Louise Susan; Lymn, Joanne; Hughes, Alun D. (2013-12)The phosphoinositol phospholipase C (PLC) family of enzymes consists of a number of isoforms, each of which has different cellular functions. PLCγ1 is primarily linked to tyrosine kinase transduction pathways, whereas PLCδ1 ... -
Making anti-thrombotic bypass vessels from selected populations of vascular smooth muscle cells
Dhanji, A.A.; Ali, F.Y.; MacKenzie, Louise Susan; Shipolini, A.; Mitchell, J.A.; Warner, T.D. (2010-06)Objectives: Vein grafts are commonly used for coronary artery bypass surgery but poor long-term patency and high failure rate make the idea of ‘designer’ bypass vessels attractive. Here we have investigated the possibility ... -
Nitric oxide-dependent vasodilation is compromised in isolated pulmonary arteries from COX knockout mice
Wright, Will; MacKenzie, Louise Susan; Kirkby, N.S.; Mitchell, Jane A. (2013-04)Cyclooxygenase (COX) has two isoforms and is essential for prostanoid synthesis. COX-1 is constitutive whilst COX-2 is induced in inflammation. Two COX products, prostacyclin (PGI2) and thromboxane (TxA2), regulate vessel ... -
Not so EEZE : the ‘EDHF’ antagonist 14, 15 Epoxyeicosa-5(z)-enoic Acid has vasodilator properties in mesenteric arteries
MacKenzie, Louise Susan; Falck, John R.; Mitchell, Jane A. (2004-12-15)P-450 metabolites, including the epoxyeicosatrienoic acids, are likely candidates for endothelial derived hyperpolarising factor (EDHF). In the present study, we confirm that the stable analogue 11-nonyloxyundec-8(Z)-enoic ... -
P-419: Phospholipase C isoforms and differentiation state of cultured human Vascular Smooth Muscle Cells.
MacKenzie, Louise Susan; Shelley, C.; Patel, M.K.; Hughes, A.D.; Lymn, Joanne (2001)Phospholipase C (PLC) plays an important role in cellular signalling. A number of isoforms of PLC exist; PLCγ1 is highly expressed in fetal and neoplastic tissue, while PLCδ1 is present in large amounts in differentiated ... -
The PPAR target gene CD36 is a rate limiting factor for the sensing of gram positive S-aureus in vascular smooth muscle cells but not in macrophages
Moreno, L.; McMaster, S.K.; Paul-Clark, M.; MacKenzie, Louise Susan; Cartwright, N.; Secher, T.; Quesniaux, V.; Ryffel, B.; Mitchell, J.A. (2008-08-01)Bacteria activate macrophages and vascular smooth muscle cells (VSMCs) resulting in induction of nitric oxide synthase (NOS)II activity. Here we show that the PPARc agonist rosiglitazone (rosi) increases the sensing of ... -
The PPARβ/δ agonist GW0742 prevents LPS-induced nitrite production in rat parenchyma but not in aorta or pulmonary arteries
Perez Diaz, Noelia; Barakat, Jehan; Perez-Vizcaino, Fernando; Moreno, Laura; MacKenzie, Louise Susan (2014-12)Peroxisome Proliferator activated receptors (PPARs) are therapeutic targets in the treatment of inflammatory lung disease. The PPARβ/δ agonist GW0742 has potent anti-inflammatory effects in the vasculature (Kapoor et al ... -
Relative expression of cyclo-oxygenase COX-1 and COX-2 in porcine endothelium subjected to chronic shear stress
Potter, Claire M.F.; Lundberg, Martina H.; MacKenzie, Louise Susan; Warboys, C.M.; Gashaw, Hime; Warner, Timothy D.; Weinberg, P.D.; Mitchell, Jane A. (2009) -
Role of COX-1 and COX-2 in the release of prostanoids in murine lung and isolated lung fibroblasts
Wright, William; Kirkby, Nick; MacKenzie, Louise Susan; Lundberg, Martina; Paul-Clark, M.J.; Warner, T.D. (2011-06)Cyclooxygenase (COX) is the first enzyme in the conversion of arachidonic acid to prostanoids. There are two isoforms of COX; COX-1, which is constitutively expressed with a homeostatic role in most tissues, and COX-2, ... -
Role of prostacyclin in pulmonary hypertension
Mitchell, Jane A.; Ahmetaj-Shala, Blerina; Kirkby, Nick; Wright, William R.; MacKenzie, Louise Susan; Reed, Dan; Mohamed, Nura (2014-12-31)Prostacyclin is a powerful cardioprotective hormone released by the endothelium of all blood vessels. Prostacyclin exists in equilibrium with other vasoactive hormones and a disturbance in the balance of these factors leads ... -
Role of RhoB in the regulation of pulmonary endothelial and smooth muscle cell responses to hypoxia
Wojciak-Stothard, B.; Zhao, L.; Oliver, E.; Dubois, O.; Wu, Y.; Kardassis, D.; Vasilaki, E.; Mitchell, J.A.; Huang, M.; MacKenzie, Louise Susan; Prendergast, G.C.; Wilkins, M.R. (2012-05-25)Rationale: RhoA and Rho kinase contribute to pulmonary vasoconstriction and vascular remodeling in pulmonary hypertension. RhoB, a protein homologous to RhoA and activated by hypoxia, regulates neoplastic growth and ... -
Role of the endothelium and COX-1 in prostacyclin generation by whole vessels stimulated with different agonists
Alyamanil, Malak; Kirkby, N.S.; MacKenzie, Louise Susan; Lundberg, M.H.; Warner, T.D.; Mitchell, J.A. (2012)Prostacyclin is an important cardioprotective hormone produced by the vascular wall, whose synthesis is dependent on cyclo-oxygenase (COX) enzymes. In healthy vessels the endothelium is thought to be the main site of ...