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dc.contributor.authorJamal, Atif
dc.contributor.authorBignell, Elaine M.
dc.contributor.authorCoutts, Robert H.A.
dc.identifier.citationJamal , A , Bignell , E M & Coutts , R H A 2010 , ' Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus ' , Virus Research , vol. 153 , no. 1 , pp. 64-70 .
dc.identifier.otherPURE: 1908974
dc.identifier.otherPURE UUID: 61fb9aa4-a5e1-418b-983f-168a4ac081e1
dc.identifier.otherPubMed: 20621139
dc.identifier.otherScopus: 77956225874
dc.description(c) 2010 Elsevier B.V. All rights reserved.
dc.description.abstractA new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in five of 390 A. fumigatus isolates screened. Analysis of purified dsRNA revealed four distinct species 3560, 3159, 3006 and 2863 base pairs in length (dsRNAs 1-4) which were cloned and sequenced. Each dsRNA contains a single open reading frame (ORF) with short 5' and 3' untranslated regions containing strictly conserved termini. The deduced 1114 amino acid (aa) protein (molecular mass=128 kDa) encoded by the dsRNA1 ORF showed homology to the RNA-dependent RNA polymerase (RdRP) of viruses belonging to the Chrysoviridae. Eight motifs characteristic of RdRPs were identified. The dsRNA2 ORF encodes the putative coat protein subunit (953aa; molecular mass=107 kDa). The dsRNA3 and dsRNA4 ORFs respectively encode putative proteins (891aa, molecular mass=99 kDa) and (847aa, molecular mass=95 kDa), both of which have significant similarity to proteins encoded by comparable chrysovirus dsRNAs. The dsRNA profile, amino acid sequence alignments, and phylogenetic analyses all indicate that AfuCV is a new species within the family Chrysoviridae.en
dc.relation.ispartofVirus Research
dc.subjectViral Proteins
dc.subjectRNA, Double-Stranded
dc.subjectCapsid Proteins
dc.subjectOpen Reading Frames
dc.subjectAmino Acid Sequence
dc.subjectSequence Analysis, DNA
dc.subjectMolecular Weight
dc.subjectCloning, Molecular
dc.subjectSequence Alignment
dc.subjectConserved Sequence
dc.subjectRNA Viruses
dc.subjectAspergillus fumigatus
dc.subjectMolecular Sequence Data
dc.subject3' Untranslated Regions
dc.subjectSequence Homology, Amino Acid
dc.subject5' Untranslated Regions
dc.subjectCluster Analysis
dc.subjectRNA Replicase
dc.titleComplete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatusen
dc.contributor.institutionHealth & Human Sciences Research Institute
dc.contributor.institutionSchool of Life and Medical Sciences
dc.contributor.institutionDepartment of Human and Environmental Sciences
dc.contributor.institutionAgriculture, Veterinary and Food Sciences
dc.contributor.institutionGeography, Environment and Agriculture
dc.contributor.institutionCrop and Environmental Protection
dc.description.statusPeer reviewed
dc.relation.schoolSchool of Life and Medical Sciences
rioxxterms.typeJournal Article/Review

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