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Browsing University of Hertfordshire by Author "Hutter, Victoria"
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Characterisation of ATP-binding cassette (ABC) transporters in the Calu-3 human bronchial epithelial cell culture model
Hutter, Victoria; Hilgendorf, Constanze; Brown, Alan; Cooper, Anne; Zann, Vanessa; Pritchard, David; Bosquillon, Cynthia (2010-10)Expression and functionality of ATP-binding cassette (ABC) transporters were assessed in Calu-3 cell monolayers cultured at an air-liquid interface. Transporter gene expression was in agreement with published data in human ... -
Co-Incubation with PPARβ/δ Agonists and Antagonists Modeled Using Computational Chemistry: Effect on LPS Induced Inflammatory Markers in Pulmonary Artery
Perez Diaz, Noelia; Lione, Lisa; Hutter, Victoria; Mackenzie, Louise Susan (MDPI Multidisciplinary Digital Publishing Institute, 2022-05-01)Peroxisome proliferator activated receptor beta/delta (PPARβ/δ) is a nuclear receptor ubiquitously expressed in cells, whose signaling controls inflammation. There are large discrepancies in understanding the complex role ... -
Co-Incubation with PPARβ/δ Agonists and Antagonists Modeled Using Computational Chemistry: Effect on LPS Induced Inflammatory Markers in Pulmonary Artery
Perez Diaz, Noelia; Lione, Lisa; Hutter, Victoria; Mackenzie, Louise Susan (2021-03-19)Peroxisome proliferator activated receptor beta/delta (PPARβ/δ) is a nuclear receptor ubiquitously expressed in cells, whose signaling controls inflammation. There are large discrepan-cies in understanding the complex role ... -
Comparison of Oral, Intranasal and Aerosol Administration of Amiodarone in Rats as a Model of Pulmonary Phospholipidosis.
Bodhaniya, Aateka; Hoffman, Ewelina; Ball, Dough; Klapwijk, Jan; Steven, Rory T.; Dexter, Alex; Bunch, Josephine; Baker, Daniel; Murnane, Darragh; Hutter, Victoria; Page, Clive; Dailey, Lea Ann; Forbes, Ben (2019-07-17)‘Foamy’ alveolar macrophages (FAM) observed in nonclinical toxicology studies during inhaled drug development may indicate drug-induced phospholipidosis, but can also derive from adaptive non-adverse mechanisms. Orally ... -
Diffusion through the ex vivo vitreal body - bovine, porcine, and ovine models are poor surrogates for the human vitreous
Shafaie, Sara; Hutter, Victoria; Brown, Marc; Cook, Michael; Chau, David (2018-10-25)The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age. To date, the viscoelastic properties of the human vitreous ... -
Digoxin net secretory transport in bronchial epithelial cell layers is not exclusively mediated by P-glycoprotein/MDR1
Hutter, Victoria; Chau, David Y.S.; Hilgendorf, Constanze; Brown, Alan; Cooper, Anne; Zann, Vanessa; Pritchard, David I.; Bosquillon, Cynthia (2014-01)The impact of P-glycoprotein (MDR1, ABCB1) on drug disposition in the lungs as well as its presence and activity in in vitro respiratory drug absorption models remain controversial to date. Hence, we characterised MDR1 ... -
The effect of dilution of fusidic acid cream and betamethasone dipropionate cream in complex extemporaneous mixes on formulation performance
Beebeejaun, Mubinah; Brown, Marc; Hutter, Victoria; Kravitz, Laura; McAuley, William (2022-08-25)The Aron regimen is an unconventional therapy which entails frequent applications of an extemporaneously prepared three component system (a topical antibiotic, a corticosteroid and an emollient), with the intention of ... -
The effect of processing variables on structural and mechanical properties of supercritical CO2 foamed scaffolds for tissue engineering
White, Lisa; Hutter, Victoria; Tai, Hong Yong; Howdle, Steve; Shakesheff, Kevin (2012-01)The porous structure of a scaffold determines the ability of bone to regenerate within this environment. In situations where the scaffold is required to provide mechanical function, balance must be achieved between optimizing ... -
The eggshell membrane : A potential biomaterial for corneal wound healing
Mensah, Rosemond A; Jo, Seung Bin; Kim, Hoon; Park, Sung-Min; Patel, Kapil D; Cho, Kyong J; Cook, Michael T; Kirton, Stewart B; Hutter, Victoria; Sidney, Laura E; Alves-Lima, Decio; Lin, Hungyen; Lee, Jung-Hwan; Kim, Hae-Won; Chau, David Ys (2021-11-30)The eggshell membrane (ESM) is an abundant resource with innate complex structure and composition provided by nature. With at least 60 million tonnes of hen eggs produced globally per annum, utilisation of this waste ... -
Evaluation of layers of the rat airway epithelial cell line RL-65 for permeability screening of inhaled drug candidates.
Hutter, Victoria; Hilgendorf, Constanze; Cooper, Anne; Zann, Vanessa; Pritchard, David; Bosquillon, Cynthia (2012-09-29)A rat respiratory epithelial cell culture system for in vitro prediction of drug pulmonary absorption is currently lacking. Such a model may however enhance the understanding of interspecies differences in inhaled drug ... -
Expression and functionality of P-glycoprotein in human bronchial epithelial cells in vitro
Hutter, Victoria; Hilgendorf, Constanze; Cooper, Anne; Zann, Vanessa; Pritchard, David; Bosquillon, Cynthia (2011-09)P-glycoprotein (P-gp) is expressed in normal tissues with barrier functions where it participates in cell defence mechanisms (Huls, M. et al. J Pharm Exp Ther 2009; 328:3-9). Its presence in the bronchial epithelium and ... -
High content analysis of in vitro alveolar macrophage responses can provide mechanistic insight for inhaled product safety assessment
Hutter, Victoria; Hopper, Sarah; Skamarauskas, John; Hoffman, Ewelina (2023-02-28)Assessing the safety of inhaled substances in the alveolar region of the lung requires an understanding of how the respired material interacts with both physical and immunological barriers. Human alveolar-like macrophages ... -
High Content Image Analysis as a Tool to Morphologically Distinguish Macrophage Activation and Determine Its Importance for Foamy Alveolar Macrophage Responses
Hoffman, Ewelina; Napieralska, Paulina; Mahendran, Rhamiya; Murnane, Darragh; Hutter, Victoria (2021-09-01)Introduction: Lung diseases are an increasing global health burden affecting millions of people worldwide. Only a few new inhaled medicines have reached the market in the last 30 years, in part due to foamy alveolar ... -
In Vitro Cell Models for Ophthalmic Drug Development Applications
Shafaie, Sara; Hutter, Victoria; Cook, Michael T.; Brown, Marc; Chau, David Y.S. (2016-04-20)Tissue engineering is a rapidly expanding field that aims to establish feasible techniques to fabricate biologically equivalent replacements for diseased and damaged tissues/organs. Emerging from this prospect is the ... -
Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment
Turner, Jan; Pound, Pandora; Owen, Carla; Hutchinson, Isobel; Hop, Marina; Chau, David Y. S.; Barrios Silva, Lady V.; Coleman, Michael; Dubourg, Audrey; Harries, Lorna W.; Hutter, Victoria; Kenna, J. Gerry; Lauschke, Volker M.; Neuhaus, Winifred; Roper, Clive; Watkins, Paul B.; Welch, Jonathan; Rego Alvarez, Laura; Taylor, Kevin (2023-07-20)New approach methodologies (NAMs) based on human biology enable the assessment of adverse biological effects of pharmaceuticals and other chemicals. Currently, however, it is unclear how NAMs should be used during drug ... -
Influence of surface geometry on the culture of human cell lines: a comparative study using flat, round-bottom and v-shaped 96 well plates
Shafaie, Sara; Hutter, Victoria; Brown, Marc; Cook, Michael; Chau, David (2017-10-30)In vitro cell based models have been invaluable tools for studying cell behaviour and for investigating drug disposition, toxicity and potential adverse effects of administered drugs. Within this drug discovery pipeline, ... -
Investigating the suitability of high content image analysis as a tool to assess the reversibility of foamy alveolar macrophage phenotypes in vitro.
Hoffman, Ewelina; Murnane, Darragh; Hutter, Victoria (2020-03-13)Many potential inhaled medicines fail during development due to the induction of a highly vacuolated or “foamy” alveolar macrophage phenotype response in pre-clinical studies. There is limited understanding if this response ... -
Longitudinal characterization of TK6 cells sequentially adapted to animal product-free, chemically defined culture medium: considerations for genotoxicity studies
Perez-Diaz, Noelia; Hoffman, Ewelina; Clements, Julie; Cruickshank, Rebecca; Doherty, Ann; Ebner, Daniel; Elloway, Joanne; Fu, Jianan; Kelsall, Joanne; Millar, Val; Saib, Ouarda; Scott, Andrew; Woods, Ian; Hutter, Victoria (2023-07-04)Introduction: In vitro approaches are an essential tool in screening for toxicity of new chemicals, products and therapeutics. To increase the reproducibility and human relevance of these in vitro assessments, it is advocated ... -
Morphometric Characterization of Rat and Human Alveolar Macrophage Cell Models and their Response to Amiodarone using High Content Image Analysis
Hoffman, Ewelina; Bodhaniya, Aateka; Ball, Doug; Klapwijk, Jan; Millar, Val; Kumar, Abhinav; Martin, Abigail; Mahendran, Rhamiya; Dailey, Lea Ann; Forbes, Ben; Hutter, Victoria (2017-12-31)Purpose. Progress to the clinic may be delayed or prevented when vacuolated or “foamy” alveolar macrophages are observed during non-clinical inhalation toxicology assessment. The first step in developing methods to study ... -
Optimising poly(lactic-co-glycolic acid) microparticle fabrication using a Taguchi orthogonal array design-of-experiment approach
Mensah, Rosemond; Kirton, Stewart; Cook, Michael T.; Styliari, Ioanna Danai; Hutter, Victoria; Chau, David (2019-09-26)The objective of this study was to identify, understand and generate a Taguchi orthogonal array model for the formation of 10–50 μm microparticles with applications in topical/ocular controlled drug delivery. Poly(lactic ...