Browsing Research publications by Author "Ward, Elaine"
Now showing items 21-36 of 36
-
Microbiota in Wheat Roots Evaluated by Cloning of ITS1/2 rDNA and Sequencing
Kwasna, Hanna; Bateman, Geoffrey L.; Ward, Elaine (2010-04)Fungi (17 species), oomycetous organisms (four species of Pythium) and a plasmodiophorid (Polymyxa graminis) were recorded in wheat roots analysed by cloning of the total ITS1/2 rDNA and sequencing of representative clones. ... -
Molecular diagnostics for fungal plant pathogens
McCartney, H. A.; Foster, S. J.; Fraaije, B. A.; Ward, Elaine (2003-02)Accurate identification of fungal phytopathogens is essential for virtually all aspects of plant pathology, from fundamental research on the biology of pathogens to the control of the diseases they cause. Although molecular ... -
The nucleotide sequence of the tnpA gene of Tn21
Ward, Elaine; Grinsted, J. (1987-02-25)The nucleotide sequence of the tnpA gene of Tn21 is presented. The traneposase encoded by this gene is exactly the same length (988 amino acids) as the Tn501 transposase (4). and shows 72% homology overall with this protein, ... -
Phylogenetic relationships among Zygomycetes from soil based on ITS1/2 rDNA sequences
Kwasna, Hanna; Ward, Elaine; Bateman, Geoffrey L. (2006-05)New information was obtained on the phylogeny of Zygomycetes. PCR-RFLP analysis showed ITS1/2 rDNA to provide appropriate markers for genetic studies on Zygomycetes at the population and species levels. The use of several ... -
Plant pathogen diagnostics : immunological and nucleic acid-based approaches
Ward, Elaine; Foster, S. J.; Fraaije, B. A.; McCartney, H. A. (2004)Accurate disease diagnosis and precise identification of any pathogens involved is an essential prerequisite for understanding plant diseases and controlling them effectively. Traditional methods of identifying plant ... -
Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase
Avila, P.; Delacruz, F.; Ward, Elaine; Grinsted, J. (1984-06)In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded ... -
The Pochonia chlamydosporia Serine Protease Gene vcp1 Is Subject to Regulation by Carbon, Nitrogen and pH : Implications for Nematode Biocontrol
Ward, Elaine; Kerry, Brian R.; Manzanilla-Lopez, Rosa H.; Mutua, Gerald; Devonshire, Jean; Kimenju, John; Hirsch, Penny R. (2012-04-27)The alkaline serine protease VCP1 of the fungus Pochonia chlamydosporia belongs to a family of subtilisin-like enzymes that are involved in infection of nematode and insect hosts. It is involved early in the infection ... -
A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum
Freeman, J.; Ward, Elaine; Calderon, C.; McCartney, A. (2002-11)The development of a polymerase chain reaction (PCR) assay for the detection of inoculum of the plant pathogenic fungus Sclerotinia sclerotiorum is described. The PCR primers were designed using nuclear ribosomal DNA ... -
Polymyxa graminis and the cereal viruses it transmits : a research challenge
Kanyuka, K.; Ward, Elaine; Adams, M. J. (2003-09)Polymyxa graminis is a eukaryotic obligate biotrophic parasite of plant roots that belongs to a poorly studied discrete taxonomic unit informally called the 'plasmodiophorids'. P. graminis is nonpathogenic, but has the ... -
RAPD-based inter- and intravarietal classification of fungi of the Gaeumannomyces-Phialophora complex
Augustin, C.; Ulrich, K.; Ward, Elaine; Werner, A. (1999-02)The suitability of randomly amplified polymorphic DNAs (RAPD) for differentiation at the varietal and intravarietal level was tested on several hundred isolates of the Gaeumannomyces-Phialophora (G-P) complex from different ... -
Relationships among Fusarium spp estimated by comparing restriction fragment length polymorphisms in polymerase chain reaction-amplified nuclear rDNA
Bateman, Geoffrey L.; Kwasna, Hanna; Ward, Elaine (1996-12)Nuclear rDNA from 120 isolates of 34 Fusarium spp. and Microdochium nivale was compared by restriction fragment length polymorphism (RFLP) analysis after polymerase chain reaction amplification. The RFLPs allowed differentiation ... -
Relationships among Fusarium spp. estimated by comparing restriction fragment length polymorphisms in polymerase chain reaction amplified ribosomal DNA
Bateman, Geoffrey L.; Kwasna, Hanna; Ward, Elaine (1997) -
A sensitive DNA-probe for the detection of polymyxa-betae in sugar-beets roots
Mutasa-Gottgens, Euphemia; Ward, Elaine; Adams, M. J.; Collier, C. R.; Chwarszynska, D. M.; Asher, M. J. C. (1993-11)A Polymyxa betae genomic DNA fragment of 1·9 kb has been cloned from infected sugar beet roots and shown to contain DNA sequences that are repeated on at least four different chromosomes. A probe generated from this fragment ... -
The use of conventional and quantitative real-time PCR assays for Polymyxa graminis to examine host plant resistance, inoculum levels and intraspecific variation
Ward, Elaine; Kanyuka, K.; Motteram, J.; Kornyukhin, D.; Adams, Michael J. (2005-03)A real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis. The assay was calibrated with zoospore ... -
Use of molecular methods for the detection of fungal spores
Ward, Elaine (Springer Nature, 2009)Traditional methods for the isolation and identification of fungal spores can be time-consuming and laborious. DNA-based methods for fungal detection can be used to detect the spores of plant-pathogenic fungi. Air borne ... -
Variation within and between Phytophthora species from rubber and citrus trees in China, determined by polymerase chain reaction using RAPDs
Zheng, F. C.; Ward, Elaine (1998-04)Variation among 39 isolates of Phytophthora a of six morphological species (P. citrophthora, P. parasitica, P. capsici, P. palmivora and P. meadii, from rubber and citrus trees, and P. colocasiae from tare) was studied ...