Browsing Research publications by Author "Coutts, Robert H.A."
Now showing items 1-20 of 100
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Additives for the enhancement of fusion and endocytosis in higher plant protoplasts : An electrophoretic study
Grout, B.W.W.; Coutts, Robert H.A. (1974-06-01)A range of additives has been employed to enhance both endocytosis and fusion in isolated higher plant protoplast systems. The effects of these compounds, at various concentrations, on the electrical properties of the ... -
Alterations in RNA and protein metabolism in uninoculated half-leaves of cowpea adjacent to tobacco necrosis virus infected halves
Coutts, Robert H.A.; Wagih, E.E. (1981-03-01)Following inoculation of half-leaves of cowpea with tobacco necrosis virus (TNV, R 1 : 1.5 19 : S S : S Fu), two major protein fractions, termed α and β appear in soluble extracts, which are absent from sham-inoculated ... -
Alterations in the soluble protein patterns of tobacco and cowpea leaves following inoculation with tobacco necrosis virus
Coutts, Robert H.A. (1978-06-01)Changes in the soluble protein composition of both tobacco and cowpea leaves following inoculation with tobacco necrosis virus (TNV R/1 : 1.5/19 : S/S : S/Fu [1]) have been investigated by the techniques of disc-electrophoresis. ... -
Analysis of the RNA of Potato yellow vein virus : Evidence for a tripartite genome and conserved 3′-terminal structures among members of the genus Crinivirus
Livieratos, I.C.; Eliasco, E.; Müller, G.; Salazar, L.F.; Olsthoorn, R.C.L.; Pleij, C.W.A.; Coutts, Robert H.A. (2004-07-01)Double-stranded RNA preparations produced from potato plants graft-inoculated with a Peruvian isolate of Potato yellow vein virus (PYVV; genus Crinivirus, family Closteroviridae) contain five RNA species denoted RNA 1, RNA ... -
Aspergillus fumigatus mycovirus causes mild hypervirulent effect on pathogenicity when tested on Galleria mellonella
Ozkan, S.; Coutts, Robert H.A. (2015-03-31)Mycoviruses are a specific group of viruses that naturally infect and replicate in fungi. The importance of mycoviruses was revealed after their effects were identified not only in economically important fungi but also in ... -
Beet pseudo-yellows virus is an authentic closterovirus
Coutts, Robert H.A.; Coffin, R.S. (1996-01)We have previously used RT-PCR to amplify a 251 base pair fragment diagnostic for beet pseudo-yellows virus (BPYV) infection from several BPYV infected samples from Europe and the USA. This has now been shown by sequencing ... -
The behaviour of tomato golden mosaic virus DNA in cultured cells isolated from systemically infected tobacco leaves
Slomka, M.J.; Buck, K.W.; Coutts, Robert H.A. (1989-03)When callus tissue was cultured from leaf pieces taken from a Nicotiana tabacum cv. Xanthi nc. plant systemically infected with tomato golden mosaic virus (TGMV), TGMV-specific DNA persisted for up to 6 months in culture. ... -
Cassava latent virus specific DNAs in mosaic diseased cassava of Nigerian origin
Coutts, Robert H.A.; Buck, K.W. (1987-09-01)Het genoom van het 'cassava latent' virus (CLV), een geminivirus, bestaat uit twee cirkelvormige, enkelstrengige DNA-moleculen nl. DNA 1 (2,78 kb) en DNA 2 (2,72 kb). DNA, verkregen uit Nicotiana benthamiana-planten, die ... -
Characterisation of multimeric DNA forms associated with tomato golden mosaic virus infection
Slomka, M.J.; Buck, K.W.; Coutts, Robert H.A. (1988-03-01)Homodimeric and trimeric double-stranded DNA forms of both components of the genome of the geminivirus, tomato golden mosaic virus have been isolated from infected Nicotiana tabacum plants and characterised. -
Characterisation of tomato golden mosaic virus as a geminivirus
Hamilton, W.D.O.; Sanders, R.C.; Coutts, Robert H.A.; Buck, K.W. (1981-08) -
Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. I : Characterization of a new species in the genus Chrysovirus
Covelli, L.; Hernández, C.; Flores, R.; Ragozzino, A.; Coutts, Robert H.A.; Di Serio, F.; Citir, A.; Açikgöz, S. (2004-11-01)Cherry chlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) display similar symptoms and are associated with a series of dsRNAs. However, a direct comparison has been lacking. Here, a side-by-side analysis confirmed ... -
Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II : Characterization of a new species in the genus Partitivirus
Coutts, Robert H.A.; Covelli, L.; Hernández, C.; Flores, R.; Ragozzino, A.; Di Serio, F.; Citir, A.; Açikgöz, S. (2004-11-01)Two dsRNAs from cherry trees affected with cherry chlorotic rusty spot (CCRS) in Italy and Amasya cherry disease (ACD) in Turkey were sequenced and found to be essentially identical. The larger dsRNA 1 (2021 or 2006 bp, ... -
A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance
Wang, Xianhong; Kotta-Loizou, Ioly; Coutts, Robert H.A.; Deng, Huifang; Han, Zhenhao; Hong, Ni; Shafik, Karim; Wang, Liping; Guo, Yashuang; Yang, Mengmeng; Xu, Wenxing; Wang, Guoping (2024-06-03)Circular single-stranded DNA (ssDNA) viruses have been rarely found in fungi, and the evolutionary and ecological relationships among ssDNA viruses infecting fungi and other organisms remain unclear. In this study, a novel ... -
The closteroviruses, capilloviruses and other similar viruses : A short review
Coffin, R.S.; Coutts, Robert H.A. (1993-08)The closteroviruses and capilloviruses are diverse groups of structurally similar, flexuous, filamentous plant viruses. However, because of a lack of information of other than a morphological nature the taxonomy of these ... -
A comparison, using dsRNA analysis, between beet soil-borne virus and some other tubular viruses isolated from sugar beet
Hutchinson, P.J.; Henry, C.M.; Coutts, Robert H.A. (1992-05)Double-stranded RNA preparations from Chenopodium quinoa leaves inoculated with two English isolates of beet soil-borne virus (BSBV), BSBV-N and BSBV-452N, a French isolate of beet necrotic yellow vein virus (BNYVV), a ... -
The complete nucleotide sequence of a totivirus from Aspergillus foetidus
Kozlakidis, Zisis; Herrero, Noemi; Coutts, Robert H.A. (2013-01)Virus infection of Aspergillus foetidus was first described in the 1970s, and the purified virus, named Aspergillus foetidus virus (AfV), contains at least two types of icosahedral particles, called AfV-fast (-F) and ... -
The complete nucleotide sequence of tobacco necrosis virus strain D
Coutts, Robert H.A.; Rigden, J.E.; Slabas, A.R.; Lomonossoff, G.P.; Wise, P.J. (1991-07)The complete sequence of the RNA genome of tobacco necrosis virus strain D (TNV-D) consisting of 3759 nucleotides has been determined. The positive strand contains five open reading frames (ORFs). The 5'proximal ORF encodes ... -
Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus
Jamal, Atif; Bignell, Elaine M.; Coutts, Robert H.A. (2010-10)A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in ... -
Complete nucleotide sequences of two dsRNAs associated with a new partitivirus infecting Aspergillus fumigatus
Bhatti, Muhammad Faraz; Bignell, Elaine M.; Coutts, Robert H.A. (2011-09) -
Conservation of the 3' terminal nucleotide sequence in five carlaviruses
Haylor, M.T.M.; Brunt, A.A.; Coutts, Robert H.A. (1990-10)