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Now showing items 11-19 of 19
The nucleotide sequence of cloned wheat dwarf virus DNA
(1985-09)
Restriction analysis and cloning of virus-specific double-stranded DNA isolated from plants infected with wheat dwarf virus (WDV) indicated that the virus genome, like that of maize streak virus (MSV), consists of a single ...
S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B : Identification of regions of potential alternative secondary structure and regulatory function
(1985-07-11)
The sensitivity of the supercoiled double-stranded form of the DNA of tanato golden mosaic virus (TGMV), a geminivirus, to the single-strand specific enzyme S1 nuclease has been demonstrated. Specific S1 cleavage sites ...
Negatively supercoiled DNA from plants infected with a single-stranded DNA virus
(1984-02-14)
A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled ...
Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components
(1983-11-11)
Linear double-stranded (ds)DNA, obtained by excision of the cloned A and B components of tomato golden mosaic virus (TGMV) from recombinant plasmids, was found to infect plants and to elicit symptoms identical to those ...
Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus
(1982-08-25)
We report the molecular cloning of the tomato golden mosaic virus (TGMV) genome in the E. coli plasmid pAT 153. The results of this work conclusively show that TGMV DNA consists of two components (designated A and B) of ...
Similarities in the soluble protein profiles of leaf tissue following either a hypersensitive reaction to virus infection or plasmolysis
(1981-03-01)
Cucumber cotyledons and cowpea leaves both react hypersensitively to tobacco necrosis virus (TNV) infection. The soluble fractions of these tissues, following virus-elicited necrosis, contained apparently novel proteins. ...
Alterations in RNA and protein metabolism in uninoculated half-leaves of cowpea adjacent to tobacco necrosis virus infected halves
(1981-03-01)
Following inoculation of half-leaves of cowpea with tobacco necrosis virus (TNV, R 1 : 1.5 19 : S S : S Fu), two major protein fractions, termed α and β appear in soluble extracts, which are absent from sham-inoculated ...
Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture
(1981-01)
Methods previously established for the propagation of cassava plants free from cassava mosaic disease have been applied to Nigerian clones. Meristem tips from diseased plants subjected to heat treatment for not less than ...