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Browsing by Author "Microbiology and Biotechnology"
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Activity of vancomycin against epidemic Clostridium difficile strains in a human gut model
Baines, Simon D.; O'Connor, Rachel; Saxton, Katie; Freeman, Jane; Wilcox, M. H. (2009)Vancomycin and metronidazole remain the only primary options for the treatment of Clostridium difficile infection (CDI). Recent reports have suggested a superior clinical response to vancomycin therapy compared with ... -
Agarose gel electrophoresis of nucleic acids
Williams, D.R; Rapley, Ralph (Humana Press, 2000)DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of ... -
Agarose Gel Electrophoresis of RNA
Slater, Robert (Humana Press, 1986) -
Alternative methods of teaching structural protein biochemistry - a matter of style
Brzeski, H.; Slater, Robert (2000) -
Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico
Adrian Martinez-Melendez, Adrian; Morfin-Otero, Rayo; Villarreal-Trevino, Licet; Baines, Simon; Camacho-Ortiz, Adrian; Garza-Gonzalez, Elvira (2021-03-10)Introduction Clostridioides difficile biofilms are believed to protect the pathogen from antibiotics, in addition to potentially contributing to recurrent infections. Methodology: Biofilm production of 102 C. difficile ... -
Antimicrobial resistance and reduced susceptibility in Clostridium difficile: potential consequences for induction, treatment, and recurrence of C. difficile infection.
Baines, Simon D.; Wilcox, M. H. (2015-07-10)Clostridium difficile infection (CDI) remains a substantial burden on healthcare systems and is likely to remain so given our reliance on antimicrobial therapies to treat bacterial infections, especially in an aging ... -
Basic molecular biology techniques
Rapley, Ralph (Royal Society of Chemistry, 2009) -
Basic techniques in molecular biology
Rapley, Ralph (Humana Press, 2005) -
Basic tools and techniques in molecular biology
Rapley, Ralph; Whitehouse, David (Wiley, 2007) -
Bayesian estimation of the true prevalence of Mycobacterium avium subsp. paratuberculosis infection in Cypriot dairy sheep and goat flocks
Liapi, M.; Leontides, L.; Kostoulas, P.; Botsaris, G.; Iacovou, Y.; Rees, C.; Georgiou, K.; Smith, G.C.; Naseby, D.C. (2011)We estimated the prevalence of Mycobacterium avium subsp. paratuberculosis (MAP) infected sheep and goats, older than 2 years, reared in the region effectively controlled by the Government of the Republic of Cyprus, in ... -
Bioluminescence-based measurement of viability of Pseudomonas aeruginosa ATCC 9027 harbouring plasmid-based lux genes under the control of constitutive promoters
Shah, Niksha; Naseby, David (2014-11)Detection of microbial contamination in pharmaceuticals, food and cosmetics has been problematic for several decades. Numerous investigations highlight the urgency for novel methods; development of bioluminescent constructs ... -
Can rapeseed oil replace olive oil as part of a Mediterranean-style diet?
Hoffman, Richard; Gerber, Mariette (2014-12)The present narrative review compares evidence from experimental, epidemiological and clinical studies of the health benefits of rapeseed oil (RO) (known as canola oil) and olive oil (OO) in order to assess whether rapeseed ... -
Can the paleolithic diet meet the nutritional needs of older people?
Hoffman, Richard (2017-01-01) -
A cellulose-based bioassay for the colorimetric detection of pathogen DNA
Saikrishnan, Deepika; Goyal, Madhu; Rossiter, Sharon; Kukol, A. (2014-12-01)Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen ... -
The changing epidemiology of Clostridium difficile infections
Freeman, J.; Bauer, M. P.; Baines, Simon D.; Corver, J.; Fawley, W. N.; Goorhuis, B.; Kuijper, E. J.; Wilcox, M. H. (2010)The epidemiology of Clostridium difficile infection (CDI) has changed dramatically during this millennium. Infection rates have increased markedly in most countries with detailed surveillance data. There have been clear ... -
Characterization of the sporulation initiation pathway of Clostridium difficile and its role in toxin production
Underwood, Sarah; Guan, Shuang; Vijayasubhash, Vinod; Baines, Simon D.; Graham, Luke; Lewis, Richard J.; Wilcox, M. H.; Stephenson, Keith (2009)Clostridium difficile is responsible for significant mortality and morbidity in the hospitalized elderly. C. difficile spores are infectious and are a major factor contributing to nosocomial transmission. The Spo0A response ... -
Circulation of Highly Drug-Resistant Clostridium difficile Ribotypes 027 and 001 in Two Tertiary-Care Hospitals in Mexico
Adrian Martinez-Melendez, Adrian; Tijerina-Rodrıguez, Laura; Morfin-Otero, Rayo; Camacho-Ortiz, Adrian; Villarreal-Trevino, Licet; Sanchez-Alanıs, Hugo; Rodrıguez-Noriega, Eduardo; Baines, Simon; Flores-Trevino, Samantha; Maldonado-Garza, Hector Jesus; Garza-Gonzalez, Elvira (2018-05-01)OBJECTIVE: To assess drug susceptibility and characterize Clostridium difficile ribotypes in isolates from two tertiary-care hospitals in Mexico. METHODS: Isolates were evaluated for genotyping, antimicrobial susceptibility ... -
Cloning of cold-inducible dehydrin-like genes from the blackcurrant (Ribes nigrum L.) using RT-PCR
Kemp, R.; Brennan, R.; Lanham, P.G.; Slater, R; Jones, H. (1997-04)A method is described for the identification of conserved genes in one plant species by using sequence information on internal motifs from well-characterized clones from another species. This sequence information is used ... -
Comparison of oritavancin versus vancomycin as treatments for clindamycin-induced Clostridium difficile PCR ribotype 027 infection in a human gut model
Baines, Simon D.; O'Connor, Rachael; Saxton, Katie; Freeman, Jane; Wilcox, Mark H. (2008)To compare the efficacy of oritavancin and vancomycin in the treatment of Clostridium difficile infection (CDI) using an in vitro human gut model. -
Comparison of planktonic and biofilm-associated communities of Clostridium difficile and indigenous gut microbiota in a triple-stage chemostat gut model
Crowther, Grace S.; Chilton, Caroline H.; Todhunter, Sharie L.; Nicholson, Scott; Freeman, Jane; Baines, Simon D.; Wilcox, Mark H. (2014-04-30)BACKGROUND: Biofilms are characteristic of some chronic or recurrent infections and this mode of growth tends to reduce treatment efficacy. Clostridium difficile infection (CDI) is associated with a high rate of recurrent ...