Browsing Research publications by Author "Ward, Elaine"
Now showing items 1-20 of 36
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Analysis of ribosomal DNA sequences of Polymyxa species and related fungi and the development of genus- and species-specific PCR primers
Ward, Elaine; Adams, M. J. (1998-08)A region of about 800 bp of ribosomal DNA has been sequenced in 10 isolates of Polymyxa and one isolate each of Plasmodiophora brassicae and the chytrid Olpidium brassicae. This region consists of about 330 bp at the 3' ... -
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine, J. A.; Shaw, M. W.; Napier, B.; Ward, Elaine; Fraaije, B. A. (2007-03)Leaf blotch, caused by Rhynchosporium secalis, was studied in a range of winter barley cultivars using a combination of traditional plant pathological techniques and newly developed multiplex and real-time polymerase chain ... -
Characterization of Polymyxa species by restriction analysis of PCR-amplified ribosomal DNA
Ward, Elaine; Adams, Michael J.; Mutasa, E. S.; Collier, C. R.; Asher, M. J. C. (1994-10)A molecular method is described to aid identification of the obligate parasite Polymyxa and discriminate between species (P. betae and P. graminis) and isolates. DNA was extracted from zoospores, resting spores and roots ... -
Comparison of Gaeumannomyces- and Phialophora-like fungal pathogens from maize and other plants using DNA methods
Ward, Elaine; Bateman, Geoffrey L. (1999-02)Several DNA-based techniques, developed for identifying and differentiating fungi in the Gaeumannomyces-Phialophora complex associated with cake-all diseases of cereals and grasses, were used to compare fungi from maize. ... -
Comparisons of isolates of Fusarium avenaceum from white lupin and other crops by pathogenicity tests, DNA analyses and vegetative compatibility tests
Satyaprasad, K.; Bateman, Geoffrey L.; Ward, Elaine (2000-04)Isolates of Fusarium avenaceum, mostly from crops of white lupin or wheat, were tested for pathogenicity on white lupin and wheat plants and compared by DNA tests and, in a limited study, vegetative compatibility. Most of ... -
Comparisons of isolates of the take-all fungus, Gaeumannomyces graminis var. tritici, from different cereal sequences using DNA probes and non-molecular methods
Bateman, Geoffrey L.; Ward, Elaine; Hornby, D.; Gutteridge, R. J. (1997-08)A mitochondrial ribosomal DNA probe (pEG34) that distinguishes two main restriction fragment length polymorphism (RFLP) types of isolate of the take-all fungus Gaeumannomyces graminis var. tritici was used to compare ... -
Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays
Calderon, C.; Ward, Elaine; Freeman, J.; McCartney, A. (2002-02)Conventional methods for detecting airborne fungal spores rely on either optical identification or culturing and can be time consuming or unreliable. A method for purifying DNA from conventional spore samplers and detecting ... -
Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays
Calderon, C.; Ward, Elaine; Freeman, J.; Foster, S. J.; McCartney, H. A. (2002-06)The potential use of DNA-based methods for detecting airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae, both damaging pathogens of oilseed rape, was investigated. A method for purifying DNA from spores ... -
Determining species diversity of microfungal communities in forest tree roots by pure-culture isolation and DNA sequencing
Kwasna, Hanna; Bateman, Geoffrey L.; Ward, Elaine (2008-09)Pure-culture isolation from roots was compared with transformation of total DNA from roots followed by sequence analysis of ITS 1/2 rDNA of representative clones as methods for determining the abundance and composition of ... -
Development of PCR for the detection of Polymyxa-betae in sugar beet roots and its application in field studies
Mutasa-Gottgens, Euphemia; Chwarszczynska, D. M.; Adams, Michael J.; Ward, Elaine; Asher, M. J. C. (1995-11)A previously cloned 1.8 kb PCR Polymyxa betae DNA fragment has been fully sequenced and used to design PCR primers for the specific detection of P. betae in sugar beet seedling roots. The primers did not amplify sequences ... -
Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America
Morales, F. J.; Ward, Elaine; Castano, M.; Arroyave, J. A.; Lozano, I.; Adams, M. J. (1999-10)A new disease of rice, known as 'entorchamiento' (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts ... -
Evidence that Polymyxa species may infect Arabidopsis thaliana
Smith, Madeleine J.; Adams, Michael J.; Ward, Elaine (2011-05)Polymyxa spp. are obligate biotrophs belonging to the plasmodiophorid group, responsible for transmitting a large number of plant viruses to many crop species. Their obligate nature makes them difficult to study. Controlled ... -
Gaeumannomyces graminis, the take-all fungus and its relatives
Freeman, J.; Ward, Elaine (2004-07)Take-all, caused by the fungus Gaeumannomyces graminis var. tritici, is the most important root disease of wheat worldwide. Many years of intensive research, reflected by the large volume of literature on take-all, has led ... -
Identification of fungi in the gaeumannomyces-phialophora complex-associated with take-all of cereals and grasses using DNA probes
Ward, Elaine; Bateman, Geoffrey L. (CAB International, 1994-01) -
Identification of fungi in the Gaeumannomycesp-Phialophora complex by RFLPS of PCR-amplified ribosomal DNAs
Ward, Elaine; Akrofi, A. Y. (1994-02)The polymerase chain reaction (PCR) was used to amplify ribosomal internal transcribed spacer and 5.8S DNA from isolates of Gaeumannomyces graminis, Phialophora graminicola and other root-infecting fungi, isolated mostly ... -
Improved polymerase chain-reaction (PCR) detection of Gaeumannomyces-graminis including a safeguard against false negatives
Ward, Elaine (1995-09)A previously reported method for polymerase chain reaction (PCR) detection of Gaeumannomyces graminis was modified to simplify it, improve its specificity and decrease the possibility of contamination of the assay. The ... -
Junction sequences generated by one-ended transposition
Motsch, S.; Schmitt, R.; Avila, P.; de la Cruz, F.; Ward, Elaine; Grinsted, J. (1985-05)In the presence of the cognate transposase. plasmids containing a single inverted repeat (IR) sequence of Tn21 or of Tn1721 can fuse efficiently with other plasmids (‘one-ended transposition’) (1, 2). The junctions across ... -
Lewia hordeicola sp. nov. from barley grain
Kwasna, Hanna; Ward, Elaine; Kosiak, Barbara (2006)Lewia hordeicola with Alternaria anamorph was isolated from barley grains in Norway. The fungus is homothallic. It produces fertile ascomata on synthetic nutrient agar (SNA) after long incubation at 4 C in the dark. On PCA ... -
Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores
Williams, R. H.; Ward, Elaine; McCartney, H. A. (2001-06)Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described, P, roqueforti spores were collected directly into Eppendorf tubes using a ... -
Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici
Freeman, J.; Ward, Elaine; Gutteridge, R.J.; Bateman, Geoffrey L. (2005-10)Field isolates (n = 144) of the wheat take-all fungus Gaeumannomyces graminis var. tritici (Ggt) were tested for sensitivity to silthiofam, a take-all-specific fungicide used as a seed treatment, and identified as A- or ...